BMRB Entry 36018

Title:
Solution structure of the first RRM domain of human spliceosomal protein SF3b49
Deposition date:
2016-09-06
Original release date:
2017-04-11
Authors:
Kuwasako, K.; Nameki, N.; Tsuda, K.; Takahashi, M.; Sato, A.; Tochio, N.; Inoue, M.; Terada, T.; Kigawa, T.; Kobayashi, N.; Shirouzu, M.; Ito, T.; Sakamoto, T.; Wakamatsu, K.; Guntert, P.; Takahashi, S.; Yokoyama, S.; Muto, Y.
Citation:

Citation: Kuwasako, Kanako; Nameki, Nobukazu; Tsuda, Kengo; Takahashi, Mari; Sato, Atsuko; Tochio, Naoya; Inoue, Makoto; Terada, Takaho; Kigawa, Takanori; Kobayashi, Naohiro; Shirouzu, Mikako; Ito, Takuhiro; Sakamoto, Taiichi; Wakamatsu, Kaori; Guntert, Peter; Takahashi, Seizo; Yokoyama, Shigeyuki; Muto, Yutaka. "Solution structure of the first RNA recognition motif domain of human spliceosomal protein SF3b49 and its mode of interaction with a SF3b145 fragment"  Protein Sci. 26, 280-291 (2017).
PubMed: 27862552

Assembly members:

Assembly members:
Splicing factor 3B subunit 4, polymer, 105 residues, 11378.703 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts411
15N chemical shifts94
1H chemical shifts657

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 105 residues - 11378.703 Da.

1   GLYSERSERGLYSERSERGLYPROILESER
2   GLUARGASNGLNASPALATHRVALTYRVAL
3   GLYGLYLEUASPGLULYSVALSERGLUPRO
4   LEULEUTRPGLULEUPHELEUGLNALAGLY
5   PROVALVALASNTHRHISMETPROLYSASP
6   ARGVALTHRGLYGLNHISGLNGLYTYRGLY
7   PHEVALGLUPHELEUSERGLUGLUASPALA
8   ASPTYRALAILELYSILEMETASPMETILE
9   LYSLEUTYRGLYLYSPROILEARGVALASN
10   LYSALASERALAHISASNLYSASNLEUSER
11   GLYPROSERSERGLY

Samples:

sample_1: human spliceosomal protein SF3b49, [U-100% 13C; U-100% 15N], 0.8 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

kujira, Kobayashi N - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks