BMRB Entry 3524

Title:
13C NMR Determination of the Tautomeric and Ionization States of Folate in Its Complexes with Lactobacillus casei Dihydrofolate Reductase
Deposition date:
1995-07-31
Original release date:
1995-07-31
Authors:
Cheung, H.; Birdsall, B.; Frenkiel, Thomas; Chau, D.; Feeney, J.
Citation:

Citation: Cheung, H.; Birdsall, B.; Frenkiel, Thomas; Chau, D.; Feeney, J.. "13C NMR Determination of the Tautomeric and Ionization States of Folate in Its Complexes with Lactobacillus casei Dihydrofolate Reductase"  Biochemistry 32, 6846-6854 (1993).

Assembly members:

Assembly members:
dihydrofolate reductase, polymer, 162 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1582   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactobacillus casei

Experimental source:

Experimental source:   Production method: not available

Data sets:
Data typeCount
1H chemical shifts26

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dihydrofolate reductase1

Entities:

Entity 1, dihydrofolate reductase 162 residues - Formula weight is not available

1   THRALAPHELEUTRPALAGLNASPARGASP
2   GLYLEUILEGLYLYSASPGLYHISLEUPRO
3   TRPHISLEUPROASPASPLEUHISTYRPHE
4   ARGALAGLNTHRVALGLYLYSILEMETVAL
5   VALGLYARGARGTHRTYRGLUSERPHEPRO
6   LYSARGPROLEUPROGLUARGTHRASNVAL
7   VALLEUTHRHISGLNGLUASPTYRGLNALA
8   GLNGLYALAVALVALVALHISASPVALALA
9   ALAVALPHEALATYRALALYSGLNHISPRO
10   ASPGLNGLULEUVALILEALAGLYGLYALA
11   GLNILEPHETHRALAPHELYSASPASPVAL
12   ASPTHRLEULEUVALTHRARGLEUALAGLY
13   SERPHEGLUGLYASPTHRLYSMETILEPRO
14   LEUASNTRPASPASPPHETHRLYSVALSER
15   SERARGTHRVALGLUASPTHRASNPROALA
16   LEUTHRHISTHRTYRGLUVALTRPGLNLYS
17   LYSALA

Samples:

sample_one:

sample_condition_set_one: pH: 5.3 na; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 17125 17310 17311 3525 4262
PDB
DBJ BAI41869
EMBL CAR87293 CAR90253 CDN23977
GB AAA25237 AER64174 AGP71178 AGP74091 EDY98474
PRF 0309272A 1107232A
REF WP_005686414 WP_005689288 WP_014569635 WP_033573062 WP_047676754
SP P00381
AlphaFold P00381