BMRB Entry 34968

Name: NMR structure of the C-terminal domain of the human SPAG1 protein
Published: 2025-12-15

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PDB ID: 9hkr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34968
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the C-terminal domain of the human SPAG1 protein

Deposition date:

2024-12-04

Original release date:

2025-12-15

Authors:

Chagot, M.; Quinternet, M.

Citation:

Citation: Chagot, M.; Quinternet, M.. "R2TP-like complexes as quaternary chaperones. A comprehensive overview to understand the dynamic R2SP complex" .

Assembly members:

Assembly members:
entity_1, polymer, 135 residues, 15517.994 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPHMEKLPIAKPNNAYEFGQ IINALSTRKDKEACAHLLAI TAPKDLPMFLSNKLEGDTFL LLIQSLKNNLIEKDPSLVYQ HLLYLSKAERFKMMLTLISK GQKELIEQLFEDLSDTPNNH FTLEDIQALKRQYEL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	648
15N chemical shifts	147
1H chemical shifts	1031

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 135 residues - 15517.994 Da.

1

GLY

PRO

HIS

MET

GLU

LYS

LEU

PRO

ILE

ALA

2

LYS

PRO

ASN

ALA

TYR

GLU

PHE

GLY

GLN

3

ILE

ASN

ALA

LEU

SER

THR

ARG

LYS

ASP

4

LYS

GLU

ALA

CYS

ALA

HIS

LEU

ALA

ILE

5

THR

ALA

PRO

LYS

ASP

LEU

PRO

MET

PHE

LEU

6

SER

ASN

LYS

LEU

GLU

GLY

ASP

THR

PHE

LEU

7

LEU

ILE

GLN

SER

LEU

LYS

ASN

LEU

8

ILE

GLU

LYS

ASP

PRO

SER

LEU

VAL

TYR

GLN

9

HIS

LEU

TYR

LEU

SER

LYS

ALA

GLU

ARG

10

PHE

LYS

MET

LEU

THR

LEU

ILE

SER

LYS

11

GLY

GLN

LYS

GLU

LEU

ILE

GLU

GLN

LEU

PHE

12

GLU

ASP

LEU

SER

ASP

THR

PRO

ASN

HIS

13

PHE

THR

LEU

GLU

ASP

ILE

GLN

ALA

LEU

LYS

14

ARG

GLN

TYR

GLU

LEU

Samples:

sample_1: SPAG1, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 150 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure calculation

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks