BMRB Entry 34864

Title:
Folded alpha helical de novo proteins from Apilactobacillus kunkeei
Deposition date:
2023-09-26
Original release date:
2024-02-14
Authors:
Celestine, C.
Citation:

Citation: Ye, Weihua; Krishna Behra, Phani Rama; Dyrhage, Karl; Seeger, Christian; Joiner, Joe; Karlsson, Elin; Andersson, Eva; Chi, Celestine; Andersson, Siv; Jemth, Per. "Folded Alpha Helical Putative New Proteins from Apilactobacillus kunkeei"  J. Mol. Biol. 436, 168490-168490 (2024).
PubMed: 38355092

Assembly members:

Assembly members:
entity_1, polymer, 65 residues, 7437.335 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts143
15N chemical shifts73
1H chemical shifts438

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 65 residues - 7437.335 Da.

1   GLYSERMETLYSLYSGLUGLULEUILEASP
2   LYSILELYSALAASNASNARGLEULEUASN
3   ALAVALVALGLNGLUMETTYRLEUASPASN
4   SERLEUASPILELYSTHRARGASPTYRTYR
5   ALASERASNILETHRSERVALARGGLNASN
6   GLYASPGLNILEILEGLNILELEUASPGLU
7   GLUGLYILEALAGLU

Samples:

sample_1: Orphan 5, [U-10% 13C; U-99% 15N], 500 uM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - chemical shift assignment

CcpNmr Analysis, CCPN - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks