BMRB Entry 34818

Name: Structural basis of aggregate binding/recognition by the AAA+ disaggregase ClpG
Published: 2023-11-06

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PDB ID: 8p66
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34818
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis of aggregate binding/recognition by the AAA+ disaggregase ClpG

Deposition date:

2023-05-25

Original release date:

2023-11-06

Authors:

Simon, B.; Hennig, J.; Mogk, A.

Citation:

Citation: Katikaridis, P.; Simon, B.; Jenne, T.; Moon, S.; Lee, C.; Hennig, J.; Mogk, A.. "Structural basis of aggregate binding by the AAA+ disaggregase ClpG." J. Biol. Chem. 299, 105336-105336 (2023).
PubMed: 37827289

Assembly members:

Assembly members:
entity_1, polymer, 54 residues, 6392.364 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MARKQCQVCGQPATVRVEAN LNGRHSTMLLCDDHYRQLVR QQKRTVWSHPQFEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	175
15N chemical shifts	57
1H chemical shifts	349

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	2

Entities:

Entity 1, unit_1 54 residues - 6392.364 Da.

1

MET

ALA

ARG

LYS

GLN

CYS

GLN

VAL

CYS

GLY

2

GLN

PRO

ALA

THR

VAL

ARG

VAL

GLU

ALA

ASN

3

LEU

ASN

GLY

ARG

HIS

SER

THR

MET

LEU

4

CYS

ASP

HIS

TYR

ARG

GLN

LEU

VAL

ARG

5

GLN

LYS

ARG

THR

VAL

TRP

SER

HIS

PRO

6

GLN

PHE

GLU

LYS

Entity 2, unit_2 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ClpG, [U-100% 13C; U-100% 15N], 0.8 mM; KCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView v5.0.4, Johnson, One Moon Scientific - chemical shift assignment

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

ARIA v1.2, Linge, O'Donoghue and Nilges - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks