BMRB Entry 34797

Title:
Solution structure of Mu3.1 from Conus mucronatus
Deposition date:
2023-02-17
Original release date:
2024-02-20
Authors:
Lushpa, V.; Mineev, K.; Vassilevski, A.; Koroev, D.
Citation:

Citation: Lushpa, V.; Mineev, K.; Vassilevski, A.; Koroev, D.. "Spatial structure of Mu 3.1 from Conus mucronatus"  .

Assembly members:

Assembly members:
entity_1, polymer, 23 residues, 2612.042 Da.

Natural source:

Natural source:   Common Name: Conus mucronatus   Taxonomy ID: 1127826   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus mucronatus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AXQCCRSAKYCQVXSCKRNX CCN

Data sets:
Data typeCount
13C chemical shifts55
1H chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 23 residues - 2612.042 Da.

1   ALAHYPGLNCYSCYSARGSERALALYSTYR
2   CYSGLNVALHYPSERCYSLYSARGASNHYP
3   CYSCYSASN

Samples:

sample_1: water 95%; D2O, 99.8% - deuterated, 5%; sodium azide 0.001%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

CARA v1.9.1.7, Keller and Wuthrich - chemical shift assignment, peak picking

TopSpin, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AVANCE III 800 MHz