BMRB Entry 34760

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34760
MolProbity Validation Chart

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NMR-STAR v3 text file.
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All files associated with the entry

Title:
CPSF73 CTD3
Deposition date:
2022-10-03
Original release date:
2023-04-25
Authors:
Thore, S.; Mackereth, C.
Citation:

Citation: Thore, S.; Raoelijaona, F.; Talenton, V.; Fribourg, S.; Mackereth, C.. "Molecular details of the CPSF73-CPSF100 C-terminal heterodimer and interaction with Symplekin"  Open Biol. 13, 230221-230221 (2023).
PubMed: 37989222

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, 8497.995 Da.

Natural source:

Natural source:   Common Name: Encephalitozoon cuniculi   Taxonomy ID: 6035   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Encephalitozoon cuniculi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET-MCN

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSGLKTKSVRLSKMGKKELL VSVLKNHFMVEKENDDIKVM NGDMMARISGDGVTGDAGIV DKINECIKKIEAIYLRD

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts69
1H chemical shifts521

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 77 residues - 8497.995 Da.

1   GLYSERGLYLEULYSTHRLYSSERVALARG
2   LEUSERLYSMETGLYLYSLYSGLULEULEU
3   VALSERVALLEULYSASNHISPHEMETVAL
4   GLULYSGLUASNASPASPILELYSVALMET
5   ASNGLYASPMETMETALAARGILESERGLY
6   ASPGLYVALTHRGLYASPALAGLYILEVAL
7   ASPLYSILEASNGLUCYSILELYSLYSILE
8   GLUALAILETYRLEUARGASP

Samples:

sample_1: CPSF73, [U-13C; U-15N], 800 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_2: CPSF73, [U-13C; U-15N], 800 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_3: CPSF73 800 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC methylsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HA(CACO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D DQF-COSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

Sparky, Goddard - chemical shift assignment, peak picking

TopSpin, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz
  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks