BMRB Entry 34736

Name: Human Interleukin-4 mutant - C3T-IL4
Published: 2023-10-16

Click here to enlarge.

PDB ID: 8a4f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34736
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Human Interleukin-4 mutant - C3T-IL4

Deposition date:

2022-06-11

Original release date:

2023-10-16

Authors:

Vaz, D.; Rodrigues, J.; Mueller, T.; Sebald, W.; Redfield, C.; Brito, R.

Citation:

Citation: Vaz, D.; Rodrigues, J.; Mueller, T.; Sebald, W.; Redfield, C.; Brito, R.. "RDC-refined NMR solution structure of IL-4 (pH 2.4)" .

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, 14987.209 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HKTDITLQEIIKTLNSLTEQ KTLCTELTVTDIFAASKNTT EKETFCRAATVLRQFYSHHE KDTRCLGATAQQFHRHKQLI RFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIM REKYSKCSS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	124
1H chemical shifts	741

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 129 residues - 14987.209 Da.

1

HIS

LYS

THR

ASP

ILE

THR

LEU

GLN

GLU

ILE

2

ILE

LYS

THR

LEU

ASN

SER

LEU

THR

GLU

GLN

3

LYS

THR

LEU

CYS

THR

GLU

LEU

THR

VAL

THR

4

ASP

ILE

PHE

ALA

SER

LYS

ASN

THR

5

GLU

LYS

GLU

THR

PHE

CYS

ARG

ALA

THR

6

VAL

LEU

ARG

GLN

PHE

TYR

SER

HIS

GLU

7

LYS

ASP

THR

ARG

CYS

LEU

GLY

ALA

THR

ALA

8

GLN

PHE

HIS

ARG

HIS

LYS

GLN

LEU

ILE

9

ARG

PHE

LEU

LYS

ARG

LEU

ASP

ARG

ASN

LEU

10

TRP

GLY

LEU

ALA

GLY

LEU

ASN

SER

CYS

PRO

11

VAL

LYS

GLU

ALA

ASN

GLN

SER

THR

LEU

GLU

12

ASN

PHE

LEU

GLU

ARG

LEU

LYS

THR

ILE

MET

13

ARG

GLU

LYS

TYR

SER

LYS

CYS

SER

Samples:

sample_1: Interleukin-4 mutant, C3T-IL4, [U-100% 15N], 1.5 ± 0.1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D HMQC-J	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_1	anisotropic	sample_conditions_1
2D 1H-15N HSQC IPAP	sample_1	isotropic	sample_conditions_1

Software:

Felix, Accelrys Software Inc. - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

Oxford instruments Home-built 600 MHz
Oxford instruments Home-built 750 MHz
Oxford instruments Home-built 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks