BMRB Entry 34731

Title:
The NMR structure of the MAX47 effector from Magnaporthe Oryzae
Deposition date:
2022-04-12
Original release date:
2023-04-18
Authors:
Lahfa, M.; Padilla, A.; de Guillen, K.; Pissarra, J.; Raji, M.; Cesari, S.; Kroj, T.; Gladieux, P.; Roumestand, C.; Barthe, P.
Citation:

Citation: Lahfa, M.; Padilla, A.; de Guillen, K.; Pissarra, J.; Raji, M.; Cesari, S.; Kroj, T.; Gladieux, P.; Roumestand, C.; Barthe, P.. "1H, 13C, 15N backbone and side-chain NMR assignments for three MAX effectors from Magnaporthe oryzae"  Biomol. NMR Assign. 16, 305-309 (2022).
PubMed: 35657473

Assembly members:

Assembly members:
entity_1, polymer, 95 residues, 10780.053 Da.

Natural source:

Natural source:   Common Name: Pyricularia oryzae   Taxonomy ID: 318829   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Pyricularia oryzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts93
1H chemical shifts574

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 95 residues - 10780.053 Da.

1   GLYPROHISMETASNASNVALMETALASER
2   SERSERSERASPTHRASPSERASPSERSER
3   PROASPARGGLYLEUSERARGMETCYSCYS
4   VALTYRLYSILEHISPROGLYGLYASNILE
5   TRPSERTHRLYSLYSGLYGLUGLNALATRP
6   PHEARGARGARGPHESERLYSTYRGLUVAL
7   METALATYRASPARGCYSASNLEUGLUTRP
8   GLYPHESERGLYLYSPROARGGLYLEUTHR
9   PHEGLUPHELEUTRPASPLYSGLUALAALA
10   ALAASPGLYTHRCYS

Samples:

sample_1: MAX47, [U-15N], 0.5 mM; sodium acetate 25 mM; DTT 2 mM

sample_2: MAX47, [U-13C; U-15N], 0.5 mM; sodium acetate 25 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0 mM; pH: 4.6; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation

CINDY v2.1, Padilla, A. - chemical shift assignment

TopSpin v3.6, Bruker Biospin - peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks