BMRB Entry 34705

Title:
Hm-AMP2
Deposition date:
2022-02-01
Original release date:
2022-11-28
Authors:
Grafskaia, E.; Pavlova, E.; Latsis, I.; Malakhova, M.; Lavrenova, V.; Ivchenkov, D.; Bashkirov, P.; Kot, E.; Mineev, K.; Arseniev, A.; Klinov, D.; Lazarev, V.
Citation:

Citation: Grafskaia, E.; Pavlova, E.; Latsis, I.; Malakhova, M.; Ivchenkov, D.; Bashkirov, P.; Kot, E.; Mineev, K.; Arseniev, A.; Klinov, D.; Lazarev, V.. "Non-toxic antimicrobial peptide Hm-AMP2 from leech metagenome proteins identified by the gradient-boosting approach"  Materials 224, 111364-. (2022).

Assembly members:

Assembly members:
entity_1, polymer, 13 residues, 1730.047 Da.

Natural source:

Natural source:   Common Name: medicinal leech   Taxonomy ID: 6421   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hirudo medicinalis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EKRWRRLIFNYFX

Data sets:
Data typeCount
13C chemical shifts40
15N chemical shifts15
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 13 residues - 1730.047 Da.

1   GLULYSARGTRPARGARGLEUILEPHEASN
2   TYRPHENH2

Samples:

sample_1: prok_2590_2 peptide 0.85 mM; dodecyl phosphocholine, [U-2H], 85 mM; sodium acetate, [U-2H], 20 mM; sodium azide 0.01 % w/w; D2O, [U-2H], 5 % v/v

sample_conditions_1: ionic strength: 20 mM; pH: 4.8; pressure: 1 atm; temperature: 303.0 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC-TOCSYsample_1isotropicsample_conditions_1

Software:

TopSpin v3.2, Bruker Biospin - collection

CARA v1.9.1.7, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA v3.98.5, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker AVANCE II 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks