BMRB Entry 34704

Title:
Solution structure of the DNA-binding minor pilin FimT from Legionella pneumophila
Deposition date:
2022-01-28
Original release date:
2022-02-25
Authors:
Braus, S.; Hospenthal, M.; Gossert, A.
Citation:

Citation: Braus, Sebastian; Short, Francesca; Holz, Stefanie; Stedman, Matthew; Gossert, Alvar; Hospenthal, Manuela. "The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation"  Nat. Commun. 13, 1065-1065 (2022).
PubMed: 35246533

Assembly members:

Assembly members:
entity_1, polymer, 125 residues, 14379.359 Da.

Natural source:

Natural source:   Common Name: Legionella pneumophila   Taxonomy ID: 446   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Legionella pneumophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts139
1H chemical shifts926

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 125 residues - 14379.359 Da.

1   ILEGLNASNASNGLUARGGLUTHRLEUVAL
2   ASNSERILELYSTHRALAILEGLNTYRSER
3   LYSILEGLNALAILEHISLEUGLYHISPRO
4   ILETYRLEULEUPROPHEGLYSERASNGLU
5   ASNTRPSERARGGLYMETVALLEUALALYS
6   LEUASNGLNTHRTHRASNLYSTHRGLULEU
7   ILEHISGLNTRPGLNTRPSERSERASNSER
8   TRPASNILEASNTRPLYSGLYVALASPSER
9   ASNHISARGILEILEILESERASNILEPRO
10   ASNARGALAMETSERASNGLYLYSPHEILE
11   LEUASNASNLYSARGTHRASNGLULYSVAL
12   VALVALTHRLEUASNARGLEUGLYARGVAL
13   ARGVALGLYGLYASN

Samples:

sample_1: FimT, [U-13C; U-15N], 580 uM; NaPi 25 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.2; pressure: 1 Pa; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D NOESY combinedsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

CcpNmr Analysis v2.51, CCPN - peak picking

CYANA v3.98.2, Guntert, Mumenthaler and Wuthrich - structure calculation

Amber v20, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - geometry optimization

TopSpin v4.07, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AVANCE III HD 900 MHz
  • Bruker AVANCE III HD 600 MHz
  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks