BMRB Entry 34701

Title:
GB1 in mammalian cells, 10 uM
Deposition date:
2022-01-15
Original release date:
2022-12-16
Authors:
Gerez, J.; Prymaczok, N.; Kadavath, H.; Gosh, D.; Butikofer, M.; Guntert, P.; Riek, R.
Citation:

Citation: Gerez, Juan; Prymaczok, Natalia; Kadavath, Harindranath; Ghosh, Dhiman; Butikofer, Matthias; Fleischmann, Yanick; Guntert, Peter; Riek, Roland. "Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression"  Commun. Biol. 5, 1322-1322 (2022).
PubMed: 36460747

Assembly members:

Assembly members:
entity_1, polymer, 57 residues, 6360.004 Da.

Natural source:

Natural source:   Common Name: Streptococcus sp.   Taxonomy ID: 1306   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus sp.

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 57 residues - 6360.004 Da.

1   METMETGLNTYRLYSLEUILELEUASNGLY
2   LYSTHRLEULYSGLYGLUTHRTHRTHRGLU
3   ALAVALASPALAALATHRALAGLULYSVAL
4   PHELYSGLNTYRALAASNASPASNGLYVAL
5   ASPGLYGLUTRPTHRTYRASPASPALATHR
6   LYSTHRPHETHRVALTHRGLU

Samples:

sample_1: GB1, [U-13C; U-15N], 10 uM

sample_conditions_1: ionic strength: 0 Not defined; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D 15N,13C-combined 1H,1H-NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v3.98.13, P. Guntert - structure calculation

OPALp v1.4, R. Koradi - refinement

XEASY, Bartels et al. - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III HD 700 MHz