BMRB Entry 34696

Title:
Llp mutant C1G, lytic conversion lipoprotein of phage T5
Deposition date:
2021-12-16
Original release date:
2023-01-02
Authors:
Degroux, S.; Mestdach, E.; Vives, C.; Le Roy, A.; Salmon, L.; Herrman, T.; Breyton, C.
Citation:

Citation: Degroux, S.; Mestdach, E.; Vives, C.; Le Roy, A.; Salmon, L.; Herrman, T.; Breyton, C.. "Llp mutant C1G, lytic conversion lipoprotein of phage T5"  .

Assembly members:

Assembly members:
entity_1, polymer, 62 residues, 7052.991 Da.

Natural source:

Natural source:   Common Name: Escherichia phage T5   Taxonomy ID: 2695836   Superkingdom: Viruses   Kingdom: not available   Genus/species: Tequintavirus Escherichia phage T5

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pMAL-p2E

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts151
15N chemical shifts55
1H chemical shifts296

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 62 residues - 7052.991 Da.

1   GLYSERTHRPHEGLYPROLYSASPILELYS
2   CYSGLUALATYRTYRMETGLNASPHISVAL
3   LYSTYRLYSALAASNVALPHEASPARGLYS
4   GLYASPMETPHELEUVALSERPROILEMET
5   ALATYRGLYSERPHETRPALAPROVALSER
6   TYRPHETHRGLUGLYASNTHRCYSGLUGLY
7   VALPHE

Samples:

sample_1: Tris 25 ± 0.2 mM; NaCl 75 ± 0.2 mM; Sol-Llp, [U-13C; U-15N], 1.024 ± 0.02 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
HAHBNHsample_1isotropicsample_conditions_1
HAHB(CO)NHsample_1isotropicsample_conditions_1
2D 15N-HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N-13C NOESYsample_1isotropicsample_conditions_1

Software:

UNIO v2.9.5, Torsten Herrmann - chemical shift assignment, data analysis

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS v1.3, Brunger - refinement

CcpNmr Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 1000 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks