BMRB Entry 34691

Title:
NMR structure of Npl3 RRM1 bound to the AUCCAA RNA
Deposition date:
2021-11-26
Original release date:
2022-10-21
Authors:
Clery, A.; Allain, F.; Moursy, A.
Citation:

Citation: Moursy, Ahmed; Clery, Antoine; Gerhardy, Stefan; Betz, Katharina; Rao, Sanjana; Mazur, Jaroslaw; Campagne, Sebastien; Beusch, Irene; Duszczyk, Malgorzata; Robinson, Mark; Panse, Vikram Govind; Allain, Frederic H-T. "RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing"  Nat. Commun. 14, 7166-7166 (2023).
PubMed: 37935663

Assembly members:

Assembly members:
entity_1, polymer, 6 residues, 1859.189 Da.
entity_2, polymer, 88 residues, 10121.476 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: unidentified plasmid

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts80
1H chemical shifts624

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 6 residues - 1859.189 Da.

1   AUCCAA

Entity 2, unit_2 88 residues - 10121.476 Da.

1   METHISHISARGGLNGLUGLYGLULEUSER
2   ASNTHRARGLEUPHEVALARGPROPHEPRO
3   LEUASPVALGLNGLUSERGLULEUASNGLU
4   ILEPHEGLYPROPHEGLYPROMETLYSGLU
5   VALLYSILELEUASNGLYPHEALAPHEVAL
6   GLUPHEGLUGLUALAGLUSERALAALALYS
7   ALAILEGLUGLUVALHISGLYLYSSERPHE
8   ALAASNGLNPROLEUGLUVALVALTYRSER
9   LYSLEUPROALALYSARGTYRARG

Samples:

sample_1: Npl3 RRM1, [U-15N], 0.9 mM

sample_2: Npl3 RRM1, [U-100% 13C; U-100% 15N], 0.9 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 0 Pa; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1
2D NOESY F1fF2fsample_2isotropicsample_conditions_1
2D NOESY F2fsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - peak picking, structure calculation

Sparky, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE 900 MHz
  • Bruker AVANCE III HD 700 MHz
  • Bruker AVANCE III HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks