BMRB Entry 34671

Name: Structure of Viscotoxin A3 from Viscum Album in the complex with DPC micelles
Published: 2021-11-28

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PDB ID: 7pvb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34671
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Viscotoxin A3 from Viscum Album in the complex with DPC micelles

Deposition date:

2021-10-01

Original release date:

2021-11-28

Authors:

Paramonov, A.; Shenkarev, Z.

Citation:

Citation: Paramonov, Alexander; Lyukmanova, Ekaterina; Tonevitsky, Alexander; Arseniev, Alexander; Shenkarev, Zakhar. "Spatial structure and oligomerization of viscotoxin A3 in detergent micelles: Implication for mechanisms of ion channel formation and membrane lysis" Biochem. Biophys. Res. Commun. 585, 22-28 (2021).
PubMed: 34781057

Assembly members:

Assembly members:
entity_1, polymer, 46 residues, 4842.626 Da.

Natural source:

Natural source: Common Name: European mistletoe Taxonomy ID: 3972 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Viscum album

Experimental source:

Experimental source: Production method: .

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KSCCPNTTGRNIYNACRLTG APRPTCAKLSGCKIISGSTC PSDYPK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	295

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 46 residues - 4842.626 Da.

1

LYS

SER

CYS

PRO

ASN

THR

GLY

ARG

2

ASN

ILE

TYR

ASN

ALA

CYS

ARG

LEU

THR

GLY

3

ALA

PRO

ARG

PRO

THR

CYS

ALA

LYS

LEU

SER

4

GLY

CYS

LYS

ILE

SER

GLY

SER

THR

CYS

5

PRO

SER

ASP

TYR

PRO

LYS

Samples:

sample_1: VtA3 1.6 ± 0.1 mM; DPC, [U-100% 2H], 128 ± 1 mM

sample_2: VtA3 1.6 ± 0.1 mM; DPC, [U-100% 2H], 128 ± 1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 4.0; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_2	isotropic	sample_conditions_1
2D TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation

XEASY v3.98.13, Bartels et al. - chemical shift assignment

CARA, Keller and Wuthrich - data analysis

XEASY, Bartels et al. - peak picking

VNMR, Varian - processing

NMR spectrometers:

Varian UNITY 600 MHz