BMRB Entry 34661

Title:
Structure of SARS-CoV-2 nucleoprotein in dynamic complex with its viral partner nsp3a   PubMed: 35044811
Deposition date:
2021-08-26
Original release date:
2022-01-16
Authors:
Bessa, L.; Guseva, S.; Camacho-Zarco, A.; Salvi, N.; Blackledge, M.
Citation:

Citation: Bessa, Luiza Mamigonian; Guseva, Serafima; Camacho-Zarco, Aldo; Salvi, Nicola; Maurin, Damien; Perez, Laura Marino; Botova, Maiia; Malki, Anas; Nanao, Max; Jensen, Malene Ringkjobing; Ruigrok, Rob; Blackledge, Martin. "The intrinsically disordered SARS-CoV-2 nucleoprotein in dynamic complex with its viral partner nsp3a"  Sci. Adv. 8, eabm4034-eabm4034 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, 10893.183 Da.
entity_2, polymer, 73 residues, 7710.649 Da.

Natural source:

Natural source:   Common Name: 2019-nCoV, SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: 2019-nCoV, SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts156
1H chemical shifts593

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 96 residues - 10893.183 Da.

1   GLNGLYTYRLYSSERVALASNILETHRPHE
2   GLULEUASPGLUARGILEASPLYSVALLEU
3   ASNGLULYSCYSSERALATYRTHRVALGLU
4   LEUGLYTHRGLUVALASNGLUPHEALACYS
5   VALVALALAASPALAVALILELYSTHRLEU
6   GLNPROVALSERGLULEULEUTHRPROLEU
7   GLYILEASPLEUASPGLUTRPSERMETALA
8   THRTYRTYRLEUPHEASPGLUSERGLYGLU
9   PHELYSLEUALASERHISMETTYRCYSSER
10   PHETYRPROPROASPGLU

Entity 2, unit_2 73 residues - 7710.649 Da.

1   ARGASNSERSERARGASNSERTHRPROGLY
2   SERSERARGGLYTHRSERPROALAARGMET
3   ALAGLYASNGLYGLYASPALAALALEUALA
4   LEULEULEULEUASPARGLEUASNGLNLEU
5   GLUSERLYSMETSERGLYLYSGLYGLNGLN
6   GLNGLNGLYGLNTHRVALTHRLYSLYSSER
7   ALAALAGLUALASERLYSLYSPROARGGLN
8   LYSARGTHR

Samples:

sample_1: sN3, [U-15N], 500 uM; sUbl1 550 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_2: sN3, [U-13C; U-15N], 500 uM; sUbl1 550 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_3: sN3 550 uM; sUbl1, [U-15N], 500 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_4: sN3 550 uM; sUbl1, [U-13C; U-15N], 500 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_5: sN3 550 uM; sUbl1, 100% A220I Cd1 13C, 500 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_6: sN3 500 uM; sUbl1 550 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_7: sN3(S235F), [U-13C; U-15N], 500 uM; sUbl1 550 uM; sodium phosphate 50 mM; sodium chloride 250 mM; DTT 2 mM

sample_conditions_1: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_4: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_5: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_6: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_7: ionic strength: 250 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_6isotropicsample_conditions_6
15N edited 3D NOESY-HSQCsample_1isotropicsample_conditions_1
13C edited 3D NOESY-HSQCsample_2isotropicsample_conditions_2
13C edited 3D NOESY-HSQCsample_4isotropicsample_conditions_4
13C edited NOESY-HSQCsample_5isotropicsample_conditions_5
15N edited 3D NOESY-HSQCsample_3isotropicsample_conditions_3
13C edited 3D NOESY-HSQCsample_7isotropicsample_conditions_7

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMRFAM-SPARKY, Lee, W., Tonelli, M., and Markley, J.L. - data analysis

NMR spectrometers:

  • Bruker AVANCE III HD 950 MHz
  • Bruker AVANCE III HD 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks