BMRB Entry 34607

Title:
periplasmic domain of Vibrio cholerae ToxR
Deposition date:
2021-02-24
Original release date:
2021-04-05
Authors:
Gubensaek, N.; Wagner, G.; Zangger, K.
Citation:

Citation: Gubensak, N.; Wagner, G.; Schrank, E.; Falsone, F.; Berger, T.; Pavkov-Keller, T.; Reidl, J.; Zangger, K.. "The periplasmic domains of Vibriocholerae ToxR and ToxS are forming a strong heterodimeric complex independent on the redox state of ToxR cysteines."  Mol. Microbiol. 115, 1277-1291 (2021).
PubMed: 33368680

Assembly members:

Assembly members:
entity_1, polymer, 106 residues, 11649.107 Da.

Natural source:

Natural source:   Common Name: Vibrio cholerae   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts229
15N chemical shifts64
1H chemical shifts435

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 106 residues - 11649.107 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERPROSERGLNTHRSERPHELYSPRO
3   LEUTHRVALVALASPGLYVALALAVALASN
4   METPROASNASNHISPROASPLEUSERASN
5   TRPLEUPROSERILEGLULEUCYSVALLYS
6   LYSTYRASNGLULYSHISTHRGLYGLYLEU
7   LYSPROILEGLUVALILEALATHRGLYGLY
8   GLNASNASNGLNLEUTHRLEUASNTYRILE
9   HISSERPROGLUVALSERGLYGLUASNILE
10   THRLEUARGILEVALALAASNPROASNASP
11   ALAILELYSVALCYSGLU

Samples:

sample_1: ToxRp, [U-13C; U-15N], 400 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P., Guntert, Mumenthaler and Wuthrich - chemical shift calculation, refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks