BMRB Entry 34602

Title:
NMR structure of Rv1813c from Mycobacterium tuberculosis
Deposition date:
2021-02-11
Original release date:
2021-03-26
Authors:
Barthe, P.; Cohen-Gonsaud, M.
Citation:

Citation: Martin, Marianne; deVisch, Angelique; Boudehen, Yves-Marie; Barthe, Philippe; Gutierrez, Claude; Turapov, Obolbek; Aydogan, Talip; Heriaud, Laurene; Gracy, Jerome; Neyrolles, Olivier; Mukamolova, Galina; Letourneur, Francois; Cohen-Gonsaud, Martin. "A Mycobacterium tuberculosis Effector Targets Mitochondrion, Controls Energy Metabolism, and Limits Cytochrome c Exit"  Microbiol. Spectr. 11, e0106623-e0106623 (2023).
PubMed: 37036353

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, 12714.432 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 83332   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts124
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 120 residues - 12714.432 Da.

1   GLYPROHISMETPROTHRVALASPALAHIS
2   LEUALAASNGLYSERMETSERGLUVALMET
3   METSERGLUILEALAGLYLEUPROILEPRO
4   PROILEILEHISTYRGLYALAILEALATYR
5   ALAPROSERGLYALASERGLYLYSALATRP
6   HISGLNARGTHRPROALAARGALAGLUGLN
7   VALALALEUGLULYSCYSGLYASPLYSTHR
8   CYSLYSVALVALSERARGPHETHRARGCYS
9   GLYALAVALALATYRASNGLYSERLYSTYR
10   GLNGLYGLYTHRGLYLEUTHRARGARGALA
11   ALAGLUASPASPALAVALASNARGLEUGLU
12   GLYGLYARGILEVALASNTRPALACYSASN

Samples:

sample_1: Rv1813c, [U-15N], 1.0 mM

sample_2: Rv1813c, [U-13C; U-15N], 0.9 mM

sample_3: Rv1813c 0.8 mM

sample_conditions_1: ionic strength: 150 mM; pH: 5.6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACOsample_2isotropicsample_conditions_1
2D NOESYsample_3isotropicsample_conditions_1
2D TOCSYsample_3isotropicsample_conditions_1
2D DQF-COSYsample_3isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CINDY, Padilla - chemical shift assignment

Gifa, Delsuc - peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks