BMRB Entry 34567

Title:
Transmembrane helix of tumor necrosis factor alpha in trifluorethanol
Deposition date:
2020-10-28
Original release date:
2020-12-01
Authors:
Guschtschin-Schmidt, N.; Muhle-Goll, C.
Citation:

Citation: Spitz, C.; Schlosser, C.; Guschtschin-Schmidt, N.; Stelzer, W.; Menig, S.; Gotz, A.; Haug-Kroper, M.; Scharnagl, C.; Langosch, D.; Muhle-Goll, C.; Fluhrer, R.. "Increased helical flexibility of the TNFalpha transmembrane domain enhances non-canonical shedding by SPPL2a"  iScience 23, 101775-101775 (2020).

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, 3701.497 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RRCLFLSLFSFLIVAGATTL FCLLHFGVIGPQR

Data sets:
Data typeCount
13C chemical shifts92
1H chemical shifts190

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 33 residues - 3701.497 Da.

1   ARGARGCYSLEUPHELEUSERLEUPHESER
2   PHELEUILEVALALAGLYALATHRTHRLEU
3   PHECYSLEULEUHISPHEGLYVALILEGLY
4   PROGLNARG

Samples:

sample_1: TNFA_WT_TM 0.5 ± 0.1 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

TopSpin v3.2, Bruker Biospin - processing

CcpNmr Analysis v2.4.1, CCPN - chemical shift assignment

CcpNmr Analysis, CCPN - peak picking

ARIA v2, Linge, O'Donoghue and Nilges - structure calculation

NMR spectrometers:

  • Bruker AVANCE 600 MHz