BMRB Entry 34558

Title:
Capra hircus Cathelicidin-1 (dodecylphosphocholine)
Deposition date:
2020-09-10
Original release date:
2021-09-07
Authors:
Myshkin, M.; Mironov, P.; Shenkarev, Z.
Citation:

Citation: Bolosov, Ilia; Panteleev, Pavel; Sychev, Sergei; Sukhanov, Stanislav; Mironov, Pavel; Myshkin, Mikhail Yu; Shenkarev, Zakhar; Ovchinnikova, Tatiana. "Dodecapeptide Cathelicidins of Cetartiodactyla: Structure, Mechanism of Antimicrobial Action, and Synergistic Interaction With Other Cathelicidins"  Front. Microbiol. 12, 725526-725526 (2021).
PubMed: 34484167

Assembly members:

Assembly members:
entity_1, polymer, 12 residues, 1508.920 Da.

Natural source:

Natural source:   Common Name: goat   Taxonomy ID: 9925   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Capra hircus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RICQFVLIRVCR

Data sets:
Data typeCount
15N chemical shifts29
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_21
3unit_31
4unit_41

Entities:

Entity 1, unit_1 12 residues - 1508.920 Da.

1   ARGILECYSGLNPHEVALLEUILEARGVAL
2   CYSARG

Samples:

sample_1: Capra hircus Cathelicidin-1, [U-15N], 0.96 mM; DPC, [U-99% 2H], 1.6%

sample_2: DPC, [U-99% 2H], 1.6%; Capra hircus Cathelicidin-1 (dodecylphosphocholine) 0.336 mM

sample_conditions_1: ionic strength: 0.01 mM; pH: 4.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

TopSpin, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks