BMRB Entry 34555

Title:
Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2
Deposition date:
2020-09-04
Original release date:
2021-10-01
Authors:
Kawale, A.; Kang, H.; Sattler, M.
Citation:

Citation: Kawale, A.; Kang, H.; Sattler, M.. "Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2"  .

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 8907.245 Da.

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts87
1H chemical shifts552

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 81 residues - 8907.245 Da.

1   ALAMETGLYASNLYSILETYRVALGLYGLY
2   LEUPROTHRCYSLEUASNGLNASPGLNVAL
3   LYSGLULEULEUGLNSERPHEGLYGLULEU
4   LYSGLYLEUASNLEUVALMETASPTHRASN
5   THRASNLEUASNLYSGLYPHEALAPHEPHE
6   GLUTYRCYSASPPROSERVALTHRASPHIS
7   ALAILEALAGLYLEUHISGLYMETLEULEU
8   GLYASPARGARGLEUVALVALGLNARGSER
9   ILE

Samples:

sample_1: LS2 RRM2, [U-13C; U-15N], 0.5 mM; potassium phosphate pH 6.5 0.02 mM; sodium chloride 0.05 mM; DTT 0.002 mM

sample_conditions_1: ionic strength: 0.07 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Sparky, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE 750 750 MHz
  • Bruker AVANCE 800 800 MHz
  • Bruker AVANCE 900 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks