BMRB Entry 34527

Name: Structure of the cysteine-rich domain of PiggyMac, a domesticated PiggyBac transposase involved in programmed genome rearrangements
Published: 2021-06-18

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PDB ID: 6zop
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34527
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the cysteine-rich domain of PiggyMac, a domesticated PiggyBac transposase involved in programmed genome rearrangements

Deposition date:

2020-07-07

Original release date:

2021-06-18

Authors:

Bessa, L.; Guerineau, M.; Moriau, S.; Lescop, E.; Bontems, F.; Mathy, N.; Guittet, E.; Bischerour, J.; Betermier, M.; Morellet, N.

Citation:

Citation: Guerineau, M.; Bessa, L.; Moriau, S.; Lescop, E.; Bontems, F.; Mathy, N.; Guittet, E.; Bischerour, J.; Betermier, M.; Morellet, N.. "The unusual structure of the PiggyMac cysteine-rich domain reveals zinc finger diversity in PiggyBac-related transposases" Mob. DNA 12, 12-12 (2021).
PubMed: 33926516

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 9673.085 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Paramecium tetraurelia Taxonomy ID: 5888 Superkingdom: Eukaryota Kingdom: not available Genus/species: Paramecium tetraurelia

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSPEFSYFAKIQPHTFI EGEEIVKCSECGNETKVFCQ ECTILKAEVVGLCHEKDTIK CQRFHEFMDFELDKNKEVID KRKG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	343
15N chemical shifts	78
1H chemical shifts	497

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	2
3	unit_3	2

Entities:

Entity 1, unit_1 84 residues - 9673.085 Da.

1

GLY

PRO

LEU

GLY

SER

PRO

GLU

PHE

SER

TYR

2

PHE

ALA

LYS

ILE

GLN

PRO

HIS

THR

PHE

ILE

3

GLU

GLY

GLU

ILE

VAL

LYS

CYS

SER

GLU

4

CYS

GLY

ASN

GLU

THR

LYS

VAL

PHE

CYS

GLN

5

GLU

CYS

THR

ILE

LEU

LYS

ALA

GLU

VAL

6

GLY

LEU

CYS

HIS

GLU

LYS

ASP

THR

ILE

LYS

7

CYS

GLN

ARG

PHE

HIS

GLU

PHE

MET

ASP

PHE

8

GLU

LEU

ASP

LYS

ASN

LYS

GLU

VAL

ILE

ASP

9

LYS

ARG

LYS

GLY

Entity 2, unit_2 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: cysteine-rich domain of PiggyMac, [U-15N; U-13C], 400 ± 1 uM

sample_conditions_1: ionic strength: 25 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCCONH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert P. - structure calculation

CcpNmr Analysis, CCPN - peak picking

NMR spectrometers:

Bruker AVANCE III HD 600 MHz
Bruker AVANCE III HD 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks