BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34522

Title: NMR structural analysis of yeast Cox13 reveals its C-terminus in interaction with ATP   PubMed: 33971868

Deposition date: 2020-06-14 Original release date: 2021-05-14

Authors: Shu, Z.; Pontus, P.; Peter, B.; Lena, M.; Pia, A.

Citation: Zhou, Shu; Pettersson, Pontus; Bjorck, Markus; Dawitz, Hannah; Brzezinski, Peter; Maler, Lena; Adelroth, Pia. "NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP"  BMC Biol. 19, 98-98 (2021).

Assembly members:
entity_1, polymer, 129 residues, 15046.146 Da.

Natural source:   Common Name: budding yeasts   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli K-12

Entity Sequences (FASTA):
entity_1: MFRQCAKRYASSLPPNALKP AFGPPDKVAAQKFKESLMAT EKHAKDTSNMWVKISVWVAL PAIALTAVNTYFVEKEHAEH REHLKHVPDSEWPRDYEFMN IRSKPFFWGDGDKTLFWNPV VNRHIEHDD

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts101
1H chemical shifts488

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_21

Entities:

Entity 1, unit_1 129 residues - 15046.146 Da.

1   METPHEARGGLNCYSALALYSARGTYRALA
2   SERSERLEUPROPROASNALALEULYSPRO
3   ALAPHEGLYPROPROASPLYSVALALAALA
4   GLNLYSPHELYSGLUSERLEUMETALATHR
5   GLULYSHISALALYSASPTHRSERASNMET
6   TRPVALLYSILESERVALTRPVALALALEU
7   PROALAILEALALEUTHRALAVALASNTHR
8   TYRPHEVALGLULYSGLUHISALAGLUHIS
9   ARGGLUHISLEULYSHISVALPROASPSER
10   GLUTRPPROARGASPTYRGLUPHEMETASN
11   ILEARGSERLYSPROPHEPHETRPGLYASP
12   GLYASPLYSTHRLEUPHETRPASNPROVAL
13   VALASNARGHISILEGLUHISASPASP

Samples:

sample_1: Cytochrome c oxidase subunit 13, Cox13, [U-15N], 0.5 mM

sample_2: Cytochrome c oxidase subunit 13, Cox13, [U-13C; U-15N], 0.5 mM

sample_3: Cytochrome c oxidase subunit 13, Cox13, 50%[15N, 13C] mixed with 50% [14N, 12C], 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY HSQCsample_1isotropicsample_conditions_1
3D backbone experimentssample_2isotropicsample_conditions_1
3D sidechain experimentssample_2isotropicsample_conditions_1
3D 15N-NOESYsample_2isotropicsample_conditions_1
3D 13C-NOESYsample_2isotropicsample_conditions_1
3D 15N-filtered/edited-NOESYsample_3isotropicsample_conditions_1
3D 13C-filtered/edited-NOESYsample_3isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

CcpNmr Analysis, CCPN - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts