BMRB Entry 34512

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34512
MolProbity Validation Chart

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Title:
Solution NMR structure of the C-terminal arm of RSV nucleoprotein
Deposition date:
2020-04-03
Original release date:
2021-04-11
Authors:
Cardone, C.; Eleouet, J.; Galloux, M.; Sizun, C.
Citation:

Citation: Cardone, C.; Eleouet, J.; Galloux, M.; Sizun, C.. "Solution NMR structure of the C-terminal arm of RSV nucleoprotein"  .

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, 3627.982 Da.

Natural source:

Natural source:   Common Name: HRSV A (strain A2)   Taxonomy ID: 11259   Superkingdom: Viruses   Kingdom: not available   Genus/species: Orthopneumovirus Human orthopneumovirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSGVINYSVLDLTAEELEAI KHQLNPKDNDVEL

Data sets:
Data typeCount
13C chemical shifts136
15N chemical shifts34
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 33 residues - 3627.982 Da.

1   GLYSERGLYVALILEASNTYRSERVALLEU
2   ASPLEUTHRALAGLUGLULEUGLUALAILE
3   LYSHISGLNLEUASNPROLYSASPASNASP
4   VALGLULEU

Samples:

sample_1: sodium phosphate, unlabeled, 20 ± 2 mM; sodium chloride, unlabeled, 100 ± 10 mM; TCEP, unlabeled, 1 ± 0.1 mM; RSV_N_Carm, [U-13C; U-15N], 1 ± 0.1 mM

sample_conditions_1: ionic strength: 133 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

TopSpin v4.0, Bruker Biospin - processing

CcpNmr Analysis v2.4, CCPN - chemical shift assignment, peak picking

TALOS v4.21, Cornilescu, Delaglio and Bax - structure calculation

CYANA v3.98, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks