BMRB Entry 34479

Title:
NMR solution structure of class IV lasso peptide felipeptin A2 from Amycolatopsis sp. YIM10
Deposition date:
2020-01-16
Original release date:
2020-11-19
Authors:
Madland, E.; Guerrero-Garzon, J.; Zotchev, S.; Aachmann, F.; Courtade, G.
Citation:

Citation: Guerrero-Garzon, Jaime Felipe; Madland, Eva; Zehl, Martin; Singh, Madhurendra; Rezaei, Shiva; Aachmann, Finn; Courtade, Gaston; Urban, Ernst; Ruckert, Christian; Busche, Tobias; Kalinowski, Jorn; Cao, Yan-Ru; Jiang, Yi; Jiang, Cheng-Lin; Selivanova, Galina; Zotchev, Sergey. "Class IV Lasso Peptides Synergistically Induce Proliferation of Cancer Cells and Sensitize Them to Doxorubicin"  iScience 23, 101785-101785 (2020).
PubMed: 33294793

Assembly members:

Assembly members:
entity_1, polymer, 17 residues, 1867.116 Da.

Natural source:

Natural source:   Common Name: Amycolatopsis sp.   Taxonomy ID: 37632   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Amycolatopsis sp.

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Streptomyces coelicolor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGGRGYEYNKQCLIFC

Data sets:
Data typeCount
13C chemical shifts45
15N chemical shifts12
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 17 residues - 1867.116 Da.

1   GLYGLYGLYGLYARGGLYTYRGLUTYRASN
2   LYSGLNCYSLEUILEPHECYS

Samples:

sample_1: Felipeptin A2 0.5 mM

sample_conditions_1: ionic strength: 0.5 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks