BMRB Entry 34453

Title:
Solution structure of MeuNaTxalpha-1 toxin from Mesobuthus Eupeus
Deposition date:
2019-11-20
Original release date:
2020-11-27
Authors:
Mineev, K.; Kuzmenkov, A.; Khusainov, G.; Arseniev, A.; Vassilevski, A.
Citation:

Citation: Mineev, K.; Kuzmenkov, A.; Khusainov, G.; Arseniev, A.; Vassilevski, A.. "Solution structure of MeuNaTxalpha-1 toxin from Mesobuthus Eupeus"  .

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, 7196.148 Da.

Natural source:

Natural source:   Common Name: Lesser Asian scorpion   Taxonomy ID: 34648   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus eupeus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts156
15N chemical shifts65
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 64 residues - 7196.148 Da.

1   VALARGASPGLYTYRILEALAASPASPLYS
2   ASNCYSALATYRPHECYSGLYARGASNALA
3   TYRCYSASPGLUGLUCYSLYSLYSLYSGLY
4   ALAGLUSERGLYTYRCYSGLNTRPALAGLY
5   GLNTYRGLYASNALACYSTRPCYSTYRLYS
6   LEUPROASPLYSVALPROILELYSVALSER
7   GLYLYSCYSASN

Samples:

sample_1: MeuNaTx1 2 ± 0.2 mM

sample_conditions_1: ionic strength: 30 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v3.98, Guntert P. - structure calculation

CARA v1.9, Keller and Wuthrich - chemical shift assignment

TopSpin v3.5, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks