BMRB Entry 34423

Title:
NMR structure of MLP124017
Deposition date:
2019-08-05
Original release date:
2019-12-13
Authors:
Barthe, P.; de Guillen, K.; Padilla, A.; Hecker, A.
Citation:

Citation: de Guillen, K.; Lorrain, C.; Tsan, P.; Barthe, P.; Petre, B.; Saveleva, N.; Rouhier, N.; Duplessis, S.; Padilla, A.; Hecker, A.. "Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds."  Sci. Rep. 9, 18084-18084 (2019).
PubMed: 31792250

Assembly members:

Assembly members:
entity_1, polymer, 157 residues, 18200.594 Da.

Natural source:

Natural source:   Common Name: Poplar leaf rust fungus   Taxonomy ID: 203908   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Melampsora larici-populina

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts344
15N chemical shifts127
1H chemical shifts852

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 157 residues - 18200.594 Da.

1   METGLULEUPROGLUSERPHEGLUPHEILE
2   LEUTHRGLUASPMETVALTHRASPLEUASP
3   VALLYSGLYLEUGLYTYRASPPHEILEASP
4   LEUVALTHRLYSSERPROASPSERVALASN
5   SERGLUHISGLULEUALAHISPHELEUGLY
6   PROHISASPPROGLUILETYRVALASNGLY
7   LYSILEGLNTHRTHRTHRALAPHELEUGLN
8   PHEPHEARGGLNGLYLEUPHELYSLYSLEU
9   LYSASPALAGLUPHEALAILEASNVALSER
10   GLYLYSVALLYSGLUGLYGLUGLYTYRLYS
11   LEUVALTRPLYSSERALAALAGLNARGSER
12   HISASPGLNLYSILEARGTRPASPGLUALA
13   GLUALATYRILETRPARGARGLYSASPGLY
14   SERCYSTRPLEUHISSERVALLYSPHEILE
15   METSERLYSALAALAPROTYRVALALAILE
16   ASPHISHISHISHISHISHIS

Samples:

sample_1: MLP124017, [U-15N], 1 mM

sample_2: MLP124017, [U-13C; U-15N], 0.6 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CINDY, Padilla - chemical shift assignment

Gifa, Delsuc - peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks