BMRB Entry 34306

Title:
Trpzip2 structure in presence of exogenous haloprotectant molecule.
Deposition date:
2018-07-31
Original release date:
2019-08-08
Authors:
Prior, S.
Citation:

Citation: Prior, S.. "Trpzip2 structure in presence of exogenous haloprotectant molecule."  .

Assembly members:

Assembly members:
entity_1, polymer, 13 residues, 1608.776 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SWTWENGKWTWKX

Data sets:
Data typeCount
13C chemical shifts40
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 13 residues - 1608.776 Da.

1   SERTRPTHRTRPGLUASNGLYLYSTRPTHR
2   TRPLYSNH2

Samples:

sample_1: Trpzip2 2 mM; potassium phosphate 50 mM; ectoine 250 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

CcpNmr Analysis, CCPN - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMR spectrometers:

  • Bruker AVANCE III 500 MHz