BMRB Entry 34149

Title:
Solution NMR structure of human GATA2 C-terminal zinc finger domain
Deposition date:
2017-06-16
Original release date:
2019-02-01
Authors:
Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.
Citation:

Citation: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.. "Solution NMR structure of human GATA2 C-terminal zinc finger domain"  .

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, 7700.792 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts178
15N chemical shifts64
1H chemical shifts352

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 68 residues - 7700.792 Da.

1   METALAHISHISHISHISHISHISSERSER
2   GLYLEUGLUVALLEUPHEGLNGLYPROARG
3   ARGALAGLYTHRCYSCYSALAASNCYSGLN
4   THRTHRTHRTHRTHRLEUTRPARGARGASN
5   ALAASNGLYASPPROVALCYSASNALACYS
6   GLYLEUTYRTYRLYSLEUHISASNVALASN
7   ARGPROLEUTHRMETLYSLYSGLU

Entity 2, entity_2 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: GATA2 Zinc Finger 2, [U-99% 13C; U-99% 15N], 10 mg/L

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 Pa; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure calculation

Analysis v2.4.1, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX500 500 MHz
  • Bruker DRX800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks