BMRB Entry 34024

Title:
Gp5.7 mutant L42A
Deposition date:
2016-07-07
Original release date:
2017-08-11
Authors:
Liu, B.; Matthews, S.
Citation:

Citation: Liu, B.; Shadrin, A.; Sheppard, C.; Mekler, V.; Xu, Y.; Severinov, K.; Matthews, S.; Wigneshweraraj, S.. "Gp5.7 mutant L42A"  to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, 7379.427 Da.

Natural source:

Natural source:   Common Name: Enterobacteria phage T7   Taxonomy ID: 10760   Superkingdom: Viruses   Kingdom: not available   Genus/species: T7virus Escherichia virus T7

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts272
15N chemical shifts67
1H chemical shifts420

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 69 residues - 7379.427 Da.

1   METSERASPTYRLEULYSVALLEUGLNALA
2   ILELYSSERCYSPROLYSTHRPHEGLNSER
3   ASNTYRVALARGASNASNALASERLEUVAL
4   ALAGLUALAALASERARGGLYHISILESER
5   CYSALATHRTHRSERGLYARGASNGLYGLY
6   ALATRPGLUILETHRALASERGLYTHRARG
7   PHELEULYSARGMETGLYGLYCYSVAL

Samples:

sample_1: entity_1 mM; sodium chloride 300 mM; sodium phosphate 50 mM

sample_conditions_1: pH: 6; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks