BMRB Entry 31075

Title:
Temperature-dependent structures of tau aggregates
Deposition date:
2023-02-11
Original release date:
2023-05-31
Authors:
El Mammeri, N.; Duan, P.; Dregni, A.; Hong, M.
Citation:

Citation: El Mammeri, N.; Duan, P.; Dregni, A.; Hong, M.. "Influence of Fibril Growth Rates on the Structure of Tau Aggregates"  .

Assembly members:

Assembly members:
entity_1, polymer, 202 residues, 21451.641 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts259
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_21
3unit_31
4unit_41
5unit_51
6unit_61
7unit_71
8unit_81
9unit_91
10unit_101

Entities:

Entity 1, unit_1 202 residues - 21451.641 Da.

1   SERSERPROGLYSERPROGLYTHRPROGLY
2   SERARGSERARGTHRPROSERLEUPROTHR
3   PROPROTHRARGGLUPROLYSLYSVALALA
4   VALVALARGTHRPROPROLYSSERPROSER
5   SERALALYSSERARGLEUGLNTHRALAPRO
6   VALPROMETPROASPLEULYSASNVALLYS
7   SERLYSILEGLYSERTHRGLUASNLEULYS
8   HISGLNPROGLYGLYGLYLYSVALGLNILE
9   ILEASNLYSLYSLEUASPLEUSERASNVAL
10   GLNSERLYSCYSGLYSERLYSASPASNILE
11   LYSHISVALPROGLYGLYGLYSERVALGLN
12   ILEVALTYRLYSPROVALASPLEUSERLYS
13   VALTHRSERLYSCYSGLYSERLEUGLYASN
14   ILEHISHISLYSPROGLYGLYGLYGLNVAL
15   GLUVALLYSSERGLULYSLEUASPPHELYS
16   ASPARGVALGLNSERLYSILEGLYSERLEU
17   ASPASNILETHRHISVALPROGLYGLYGLY
18   ASNLYSLYSILEGLUTHRHISLYSLEUTHR
19   PHEARGGLUASNALALYSALALYSTHRASP
20   HISGLYALAGLUILEVALTYRLYSSERPRO
21   VALVAL

Samples:

sample_1: P2R tau, [U-13C; U-15N], 5 uM

sample_conditions_1: ionic strength: 135 mM; pH: 7.4; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D NCAsample_1anisotropicsample_conditions_1
2D CCsample_1anisotropicsample_conditions_1
3D NCACXsample_1anisotropicsample_conditions_1
3D NCOCXsample_1anisotropicsample_conditions_1
3D CCCsample_1anisotropicsample_conditions_1

Software:

CcpNmr Analysis, CCPN - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

TopSpin, Bruker Biospin - data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE II 800 MHz