BMRB Entry 31064

Title:
Human diaphanous inhibitory domain bound to diaphanous autoregulatory domain
Deposition date:
2022-12-12
Original release date:
2023-10-18
Authors:
Ramirez, L.; Theophall, G.; Premo, A.; Manigrasso, M.; Yepuri, G.; Burz, D.; Ramasamy, R.; Schmidt, A.; Shekhtman, A.
Citation:

Citation: Theophall, Gregory; Ramirez, Lisa; Premo, Aaron; Reverdatto, Sergey; Manigrasso, Michaele; Yepuri, Gautham; Burz, David; Ramasamy, Ravichandran; Schmidt, Ann Marie; Shekhtman, Alexander. "Disruption of the Productive Encounter Complex Results in Dysregulation of DIAPH1 Activity"  J. Biol. Chem. 299, 105342-105342 (2023).
PubMed: 37832872

Assembly members:

Assembly members:
entity_1, polymer, 256 residues, 29116.410 Da.
entity_2, polymer, 29 residues, 3161.510 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data typeCount
13C chemical shifts463
15N chemical shifts253
1H chemical shifts1706

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 256 residues - 29116.410 Da.

1   METGLYSERSERGLUARGSERHISHISHIS
2   HISHISHISSERGLYSERGLUSERLYSSER
3   ALAMETMETTYRILEGLNGLULEUARGSER
4   GLYLEUARGASPMETPROLEULEUSERCYS
5   LEUGLUSERLEUARGVALSERLEUASNASN
6   ASNPROVALSERTRPVALGLNTHRPHEGLY
7   ALAGLUGLYLEUALASERLEULEUASPILE
8   LEULYSARGLEUHISASPGLULYSGLUGLU
9   THRALAGLYSERTYRASPSERARGASNLYS
10   HISGLUILEILEARGCYSLEULYSALAPHE
11   METASNASNLYSPHEGLYILELYSTHRMET
12   LEUGLUTHRGLUGLUGLYILELEULEULEU
13   VALARGALAMETASPPROALAVALPROASN
14   METMETILEASPALAALALYSLEULEUSER
15   ALALEUCYSILELEUPROGLNPROGLUASP
16   METASNGLUARGVALLEUGLUALAMETTHR
17   GLUARGALAGLUMETASPGLUVALGLUARG
18   PHEGLNPROLEULEUASPGLYLEULYSSER
19   GLYTHRTHRILEALALEULYSVALGLYCYS
20   LEUGLNLEUILEASNALALEUILETHRPRO
21   ALAGLUGLULEUASPPHEARGVALHISILE
22   ARGSERGLULEUMETARGLEUGLYLEUHIS
23   GLNVALLEUGLNASPLEUARGGLUILEGLU
24   ASNGLUASPMETARGVALGLNLEUASNVAL
25   PHEASPGLUGLNGLYGLUGLUASPSERTYR
26   ASPLEULYSGLYARGLEU

Entity 2, unit_2 29 residues - 3161.510 Da.

1   ASPGLUTHRGLYVALLEUASPSERLEULEU
2   GLUALALEUGLNSERGLYALAALAPHEARG
3   ARGLYSARGGLYPROARGGLNALAASN

Samples:

sample_1: Diaphanous inhibitory domain, [U-15N], 300 uM; Diaphanous autoregulatory domain 500 uM

sample_2: Diaphanous inhibitory domain, [U-15N], 300 uM; Diaphanous autoregulatory domain, [U-13C], 500 uM

sample_3: Diaphanous inhibitory domain, [U-100% 13C; U-100% 15N], 300 uM; Diaphanous autoregulatory domain 500 uM

sample_4: Diaphanous inhibitory domain 300 uM; Diaphanous autoregulatory domain, [U-100% 13C; U-100% 15N], 500 uM

sample_5: Diaphanous inhibitory domain 300 uM; Diaphanous autoregulatory domain, [U-100% 15N], 300 uM

sample_conditions_1: ionic strength: 140 mM; pH: 7.5; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_5isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
3D HN(CO)CAsample_4isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D HBHA(CO)NHsample_4isotropicsample_conditions_1
H(CCH)-TOCSYsample_4isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

YASARA, Krieger - refinement

NMR spectrometers:

  • Bruker Ascend 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks