BMRB Entry 31059
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31059
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Duan, P.; Dregni, A.; Mammeri, N.; Hong, M.. "Structure of Untwisted Filaments of a Tiny Tau that Encompasses the Alzheimer's Tau b-sheet Core" .
Assembly members:
entity_1, polymer, 95 residues, 10187.614 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: IKHVPGGGSVQIVYKPVDLS
KVTSKCGSLGNIHHKPGGGQ
VEVKSEKLDFKDRVQSKIGS
LDNITHVPGGGNKKIETHKL
TFRENAKAKTDHGAE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 233 |
| 15N chemical shifts | 51 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
| 2 | unit_2 | 1 |
| 3 | unit_3 | 1 |
| 4 | unit_4 | 1 |
| 5 | unit_5 | 1 |
Entities:
Entity 1, unit_1 95 residues - 10187.614 Da.
| 1 | ILE | LYS | HIS | VAL | PRO | GLY | GLY | GLY | SER | VAL | ||||
| 2 | GLN | ILE | VAL | TYR | LYS | PRO | VAL | ASP | LEU | SER | ||||
| 3 | LYS | VAL | THR | SER | LYS | CYS | GLY | SER | LEU | GLY | ||||
| 4 | ASN | ILE | HIS | HIS | LYS | PRO | GLY | GLY | GLY | GLN | ||||
| 5 | VAL | GLU | VAL | LYS | SER | GLU | LYS | LEU | ASP | PHE | ||||
| 6 | LYS | ASP | ARG | VAL | GLN | SER | LYS | ILE | GLY | SER | ||||
| 7 | LEU | ASP | ASN | ILE | THR | HIS | VAL | PRO | GLY | GLY | ||||
| 8 | GLY | ASN | LYS | LYS | ILE | GLU | THR | HIS | LYS | LEU | ||||
| 9 | THR | PHE | ARG | GLU | ASN | ALA | LYS | ALA | LYS | THR | ||||
| 10 | ASP | HIS | GLY | ALA | GLU |
Samples:
sample_1: Tau (297-391), [U-100% 13C; U-100% 15N], 50 % w/w; MgCl2 200 mM
sample_2: Tau (297-391), 50% UCN diluted with NA, 50 % w/w; MgCl2 200 mM
sample_3: Tau (297-391), [1,3-13C]glycerol, U-15N, 50 % w/w; MgCl2 200 mM
sample_conditions_1: ionic strength: 0.61 M; pH: 5.5; pressure: 1 atm; temperature: 287 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-detected NCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D CONCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-detected CORD | sample_2 | isotropic | sample_conditions_1 |
| 2D 13C-detected long, CORD | sample_2 | isotropic | sample_conditions_1 |
| 2D 13C-detected long, CORD | sample_3 | isotropic | sample_conditions_1 |
Software:
TopSpin v4.1.1, Bruker Biospin - processing
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation
CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE NEO 900 MHz
- Bruker AVANCE 800 MHz