BMRB Entry 30948

Title:
NMR Solution Structure of hGal(1-12)KK, a solubility-tagged truncation of the human neuropeptide galanin
Deposition date:
2021-09-08
Original release date:
2022-05-17
Authors:
Kraichely, K.; Mendoza, E.; Parnham, S.; Giuliano, M.
Citation:

Citation: Kraichely, K.; Clinkscales, S.; Hendy, C.; Mendoza, E.; Parnham, S.; Giuliano, M.. "Minimal Increments of Hydrophobic Collapse within the N-Terminus of the Neuropeptide Galanin"  Biochemistry 61, 1151-1166 (2022).
PubMed: 35622960

Assembly members:

Assembly members:
entity_1, polymer, 19 residues, 1841.099 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GWTLNSAGYLLGPHAVGKX

Data sets:
Data typeCount
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 19 residues - 1841.099 Da.

1   GLYTRPTHRLEUASNSERALAGLYTYRLEU
2   LEUGLYPROHISALAVALGLYLYSNH2

Samples:

sample_1: hGal(1-17)K 2 mM; sodium acetate, D3, 4.14 mM; acetic acid, D4, 5.86 mM

sample_conditions_1: ionic strength: 4.14 mM; pH: 4.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1H acquisitionsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al., Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

MestreLab (Mnova / MestReNova / MestReC), MestreLab (Mnova / MestReNova / MestReC) - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE II 600 MHz