BMRB Entry 30874

Title:
Structure and Interactions of DED1 of human cFLIP
Deposition date:
2021-03-03
Original release date:
2021-11-28
Authors:
Panaitiu, A.; Basiashvili, T.; Mierke, D.; Pellegrini, M.
Citation:

Citation: Panaitiu, A.; Basiashvili, T.; Mierke, D.; Pellegrini, M.. "An engineered construct of cFLIP provides insight into DED1 structure and interactions"  Structure 30, 229-239 (2021).
PubMed: 34800372

Assembly members:

Assembly members:
entity_1, polymer, 105 residues, 12425.535 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET30b

Data sets:
Data typeCount
13C chemical shifts662
15N chemical shifts189
1H chemical shifts1388

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 105 residues - 12425.535 Da.

1   METHISHISHISHISHISHISMETSERALA
2   GLUVALILEHISGLNVALGLUGLUALALEU
3   SERSERSERGLULEUTHRGLULEULYSTYR
4   LEUCYSLEUGLYARGVALGLYLYSARGLYS
5   LEUGLUARGVALGLNSERGLYVALARGASP
6   LEULEUASPILELEUARGGLUARGGLYLYS
7   LEUSERVALGLYASPLEUALAGLULEULEU
8   TYRARGVALARGARGPHEASPLEULEULYS
9   ARGILELEULYSMETASPARGLYSALAVAL
10   GLUTHRHISLEULEUARGASNPROHISLEU
11   VALSERASPTYRARG

Samples:

sample_1: entity_1, [U-13C; U-15N], 300 uM; sodium succinate 20 mM; sodium chloride 250 mM; DTT 3 mM; pefabloc 0.2 mM

sample_conditions_1: ionic strength: 0.27 M; pH: 5.8; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

CS-ROSETTA, Shen, Vernon, Baker and Bax - structure calculation

TopSpin v3.2, Bruker Biospin - collection

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - peak picking

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks