BMRB Entry 30847

Title:
NMR structure of Beta-KTx14.3
Deposition date:
2021-01-21
Original release date:
2022-02-04
Authors:
Carranza-Gonzalez, L.; Titaux-Delgado, G.; del Rio-Portilla, J.
Citation:

Citation: Titaux-Delgado, G.; Lopez-Giraldo, A.; Carrillo, E.; Cofas-Vargas, L.; Carranza-Gonzalez, L.; Lopez-Vera, E.; Garcia-Hernandez, E.; del Rio-Portilla, J.. "Beta-KTx14.3, a scorpion toxin, blocks the human potassium channel KCNQ1"  Biochim. Biophys. Acta Proteins Proteom. 1871, 140906-140906 (2023).
PubMed: 36918120

Assembly members:

Assembly members:
entity_1, polymer, 48 residues, 5271.080 Da.

Natural source:

Natural source:   Common Name: Chinese swimming scorpion   Taxonomy ID: 172552   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lychas mucronatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IAGVADLNNMSQLGCPFIEK WCEDHCESKKQVGKCENFDC SCVKLGGK

Data sets:
Data typeCount
1H chemical shifts257

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 48 residues - 5271.080 Da.

1   ILEALAGLYVALALAASPLEUASNASNMET
2   SERGLNLEUGLYCYSPROPHEILEGLULYS
3   TRPCYSGLUASPHISCYSGLUSERLYSLYS
4   GLNVALGLYLYSCYSGLUASNPHEASPCYS
5   SERCYSVALLYSLEUGLYGLYLYS

Samples:

sample_1: Beta-KTx14.3 0.8 ± 0.02 mg/mL

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 289 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker AMX 700 MHz