BMRB Entry 30842

Title:
SARS-CoV-2 spike Protein TM domain
Deposition date:
2021-01-10
Original release date:
2021-06-18
Authors:
Fu, Q.; Chou, J.
Citation:

Citation: Fu, Q.; Chou, J.. "A Trimeric Hydrophobic Zipper Mediates the Intramembrane Assembly of SARS-CoV-2 Spike."  J. Am. Chem. Soc. 143, 8543-8546 (2021).
PubMed: 34086443

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, 2200.703 Da.

Natural source:

Natural source:   Common Name: 2019-nCoV   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: WLGFIAGLIAIVLVTILLSS T

Data sets:
Data typeCount
13C chemical shifts41
15N chemical shifts20
1H chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_21
3unit_31

Entities:

Entity 1, unit_1 21 residues - 2200.703 Da.

1   TRPLEUGLYPHEILEALAGLYLEUILEALA
2   ILEVALLEUVALTHRILELEULEUSERSER
3   THR

Samples:

sample_1: 1,2-dimyristoyl-sn-Glycero-3-Phosphocholine, [U-99% 2H], 55 mM; 1,2-dihexanoyl-sn-Glycero-3-Phosphocholine, [U-99% 2H], 100 mM; TRIS 20 mM; sodium azide 0.02%; sodium chloride 20 mM; Spike glycoprotein, [U-13C; U-15N], 1 mM

sample_2: 1,2-dimyristoyl-sn-Glycero-3-Phosphocholine, [U-99% 2H], 55 mM; 1,2-dihexanoyl-sn-Glycero-3-Phosphocholine, [U-99% 2H], 100 mM; TRIS 20 mM; sodium azide 0.02%; sodium chloride 20 mM; Spike glycoprotein, [U-13C; U-15N; U-2H], 1 mM

sample_3: 1,2-dimyristoyl-sn-Glycero-3-Phosphocholine 55 mM; 1,2-dihexanoyl-sn-Glycero-3-Phosphocholine 100 mM; TRIS 20 mM; sodium azide 0.02%; sodium chloride 20 mM; Spike glycoprotein, [U-15N; U-2H], 1 mM

sample_4: 1,2-dimyristoyl-sn-Glycero-3-Phosphocholine 55 mM; 1,2-dihexanoyl-sn-Glycero-3-Phosphocholine 100 mM; TRIS 20 mM; sodium azide 0.02%; sodium chloride 20 mM; Spike glycoprotein, [U-13C; U-15N; U-2H], 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlysample_3isotropicsample_conditions_1

Software:

X-PLOR NIH v2.48, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

XEASY, Bartels et al. - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks