BMRB Entry 30810

Title:
Structure of the integrin aIIb(W968V)b3 transmembrane complex
Deposition date:
2020-11-03
Original release date:
2021-09-08
Authors:
Situ, A.; Kim, J.; An, W.; Kim, C.; Ulmer, T.
Citation:

Citation: Situ, A.; Kim, J.; An, W.; Kim, C.; Ulmer, T.. "Insight Into Pathological Integrin aIIbb3 Activation From Safeguarding The Inactive State"  J. Mol. Biol. 433, 166832-166832 (2021).
PubMed: 33539882

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 4753.871 Da.
entity_2, polymer, 44 residues, 4775.867 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts140
15N chemical shifts72
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 43 residues - 4753.871 Da.

1   GLYALALEUGLUGLUARGCYSGLYILEPRO
2   ILETRPVALVALLEUVALGLYVALLEUGLY
3   GLYLEULEULEULEUTHRILELEUVALLEU
4   ALAMETTRPLYSVALGLYPHEPHELYSARG
5   ASNARGPRO

Entity 2, unit_2 44 residues - 4775.867 Da.

1   GLYGLUSERPROLYSCYSGLYPROASPILE
2   LEUVALVALLEULEUSERVALMETGLYALA
3   ILELEULEUILEGLYLEUALAALALEULEU
4   ILETRPLYSLEULEUILETHRILEHISASP
5   ARGLYSGLUPHE

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; entity_2, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 313.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

CARA, Keller and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks