BMRB Entry 30783

Title:
NMR Solution Structure of plant defensin AtD90
Deposition date:
2020-08-03
Original release date:
2020-09-07
Authors:
Harvey, P.; Craik, D.
Citation:

Citation: Bleackley, M.; Vasa, S.; Harvey, P.; Shafee, T.; Kerenga, B.; Soares Da Costa, T.; Craik, D.; Lowe, R.; Anderson, M.. "Histidine-Rich Defensins from theSolanaceaeandBrasicaceaeAre Antifungal and Metal Binding Proteins."  J. Fungi (Basel) 6, 145-145 (2020).
PubMed: 32847065

Assembly members:

Assembly members:
entity_1, polymer, 52 residues, 6061.849 Da.

Natural source:

Natural source:   Common Name: Mouse-ear cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Komagataella pastoris

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AHCDHFLGEAPVYPCKEKAC KSVCKEHYHHACKGECEYHG REVHCHCYGDYH

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts39
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 52 residues - 6061.849 Da.

1   ALAHISCYSASPHISPHELEUGLYGLUALA
2   PROVALTYRPROCYSLYSGLULYSALACYS
3   LYSSERVALCYSLYSGLUHISTYRHISHIS
4   ALACYSLYSGLYGLUCYSGLUTYRHISGLY
5   ARGGLUVALHISCYSHISCYSTYRGLYASP
6   TYRHIS

Samples:

sample_1: peptide 1.0 mg/mL

sample_2: peptide 1.0 mg/mL

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-1H ECOSYsample_2isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

CcpNmr Analysis, CCPN - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker AVANCE III HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks