BMRB Entry 30301

Title:
Solution NMR Structure of a Class I Hydrophobin from Serpula lacrymans
Deposition date:
2017-06-01
Original release date:
2018-06-01
Authors:
Kenward, C.; Langelaan, D.
Citation:

Citation: Kenward, C.; Langelaan, D.. "Solution NMR Structure of a Class I Hydrophobin from Serpula lacrymans"  .

Assembly members:

Assembly members:
Hydrophobin, polymer, 88 residues, 8440.296 Da.

Natural source:

Natural source:   Common Name: Dry rot fungus   Taxonomy ID: 578457   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Serpula Serpula lacrymans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts320
15N chemical shifts100
1H chemical shifts517

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 88 residues - 8440.296 Da.

1   GLYSERGLYSERSERGLNCYSASNALAGLY
2   PROVALGLNCYSCYSASNTHRLEUTHRSER
3   ALASERASNSERGLNALAALAGLYLEUILE
4   GLNGLNLEUGLYLEUSERGLYVALGLYALA
5   ASNVALPROVALGLYILEASNCYSASNPRO
6   ILETHRGLYILEGLYALAGLYSERGLYSER
7   SERCYSASNALAASNPROALACYSCYSASP
8   ASNVALTYRTHRASNGLYLEUGLYVALGLN
9   CYSASNPROILEASNVALASNLEU

Samples:

sample_1: MES 20 mM; Slahyd1, [U-13C; U-15N], 800 uM; sodium chloride 50 mM

sample_2: MES 20 mM; Slahyd1, [U-15N], 100 uM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

ARIA v2.3.1, Linge, O'Donoghue and Nilges - refinement, structure calculation

Analysis, CCPN - chemical shift assignment, data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR v2.4.2, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks