BMRB Entry 30073

Title:
LP2006, a handcuff-topology lasso peptide antibiotic
Deposition date:
2016-05-03
Original release date:
2017-02-23
Authors:
Tietz, Jonathan; Schwalen, Christopher; Blair, Patricia; Zakai, Uzma; Mitchell, Douglas
Citation:

Citation: Tietz, Jonathan; Schwalen, Christopher; Patel, Parth; Maxson, Tucker; Blair, Patricia; Tai, C.; Zakai, Uzma; Mitchell, Douglas. "A new genome mining tool redefines the lasso peptide biosynthetic landscape"  Nat. Chem. Biol. 13, 470-478 (2017).
PubMed: 28244986

Assembly members:

Assembly members:
Uncharacterized protein, polymer, 17 residues, 2027.247 Da.

Natural source:

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 1205910   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nocardiopsis not available

Experimental source:

Experimental source:   Production method: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Uncharacterized protein: GRPNWGFENDWSCVRVC

Data sets:
Data typeCount
13C chemical shifts17
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 17 residues - 2027.247 Da.

1   GLYARGPROASNTRPGLYPHEGLUASNASP
2   TRPSERCYSVALARGVALCYS

Samples:

sample_1: LP2006 5 ± 1 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1Hsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1anisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1

Software:

MestReNova v8.1.1, Mestrelab Research - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

TOPSPIN v1.3, Bruker Biospin - collection

XPLOR-NIH v2.36, Charles Schwieters, Marius Clore (NIH) - structure calculation

NMR spectrometers:

  • Bruker AVANCE 900 MHz