BMRB Entry 30009

Title:
Resonance assignments and NMR structure determination of tarantula toxin, F8A mutant of beta-TRTX-Pre1a
Deposition date:
2016-02-07
Original release date:
2017-02-03
Authors:
Chin, Y.K.-Y.; Wingerd, J.; Mobli, M.; Rash, L.
Citation:

Citation: Chin, Y.K.-Y.; Wingerd, J.; Mobli, M.; Rash, L.. "Resonance assignments and NMR structure determination of tarantula toxin, mu-TRTX-Pre1a"  .

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, 4253.925 Da.

Natural source:

Natural source:   Common Name: spiders   Taxonomy ID: 179873   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Psalmopoeus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLICC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SEDCLGWASRCSPKNDKCCP NYKCSSKDLWCKYKIW

Data typeCount
13C chemical shifts161
15N chemical shifts38
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 36 residues - 4253.925 Da.

1   SERGLUASPCYSLEUGLYTRPALASERARG
2   CYSSERPROLYSASNASPLYSCYSCYSPRO
3   ASNTYRLYSCYSSERSERLYSASPLEUTRP
4   CYSLYSTYRLYSILETRP

Samples:

sample_1: D2O 5%; mu-TRTX-Pre1a, [U-99% 13C; U-99% 15N], 300 uM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Rowland NMR Toolkit, Jeff Hoch - processing

TOPSPIN, Bruker Biospin - collection

Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AvanceII 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks