BMRB Entry 28117

Name: Heterogeneous ribonucleoprotein A18 RNA Recognition Motif
Published: 2021-02-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28117

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Heterogeneous ribonucleoprotein A18 RNA Recognition Motif

Deposition date:

2020-04-27

Original release date:

2021-02-13

Authors:

Roth, Braden; Coburn, Katherine; Weber, David; Varney, Kristen

Citation:

Citation: Solano-Gonzalez, Eduardo; Yu, Wenbo; Coburn, Katherine; Wilson, Gerald; Nurmemmedov, Elmar; Kesari, Santosh; Chang, Elizabeth; MacKerell, Alexander; Weber, David; Carrier, France. "Small molecules inhibitors of the heterogeneous ribonuclear protein A18 (hnRNP A18): a regulator of protein translation and an immune checkpoint" Nucleic Acids Res. 49, 1235-1246 (2021).
PubMed: 33398344

Assembly members:

Assembly members:
A18n, polymer, 93 residues, 10213.2362 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A18n: GMASDEGKLFVGGLSFDTNE QSLEQVFSKYGQISEVVVVK DRETQRSRGFGFVTFENIDD AKDAMMAMNGKSVDGRQIRV DQAGKSSDNRSRG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	350
15N chemical shifts	91
1H chemical shifts	552

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	A18n	1

Entities:

Entity 1, A18n 93 residues - 10213.2362 Da.

1

GLY

MET

ALA

SER

ASP

GLU

GLY

LYS

LEU

PHE

2

VAL

GLY

LEU

SER

PHE

ASP

THR

ASN

GLU

3

GLN

SER

LEU

GLU

GLN

VAL

PHE

SER

LYS

TYR

4

GLY

GLN

ILE

SER

GLU

VAL

LYS

5

ASP

ARG

GLU

THR

GLN

ARG

SER

ARG

GLY

PHE

6

GLY

PHE

VAL

THR

PHE

GLU

ASN

ILE

ASP

7

ALA

LYS

ASP

ALA

MET

ALA

MET

ASN

GLY

8

LYS

SER

VAL

ASP

GLY

ARG

GLN

ILE

ARG

VAL

9

ASP

GLN

ALA

GLY

LYS

SER

ASP

ASN

ARG

10

SER

ARG

GLY

Samples:

sample_A18N: A18n, [U-13C; U-15N], 0.900 mM; HEPES 18.0 mM; NaCl 45.0 mM; NaN3 2.0 mM; DTT 1.0 mM

sample_conditions_1: ionic strength: 63.000 mM; pH: 7.000; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_A18N	isotropic	sample_conditions_1
3D HNCACB	sample_A18N	isotropic	sample_conditions_1
3D HNCO	sample_A18N	isotropic	sample_conditions_1
5 (HNcaCO)	sample_A18N	isotropic	sample_conditions_1
3D C(CO)NH	sample_A18N	isotropic	sample_conditions_1
11 (HccoNH)	sample_A18N	isotropic	sample_conditions_1
12 (HccoNH)	sample_A18N	isotropic	sample_conditions_1
3D C(CO)NH	sample_A18N	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignments

nmrPipe v10.4, NIST IBBR - nmr data processing

NMR spectrometers:

Bruker DMX 600 MHz