BMRB Entry 28099

Title:
NMR Assignments of the Programmed Cell Death 5 (PDCD5) Protein from Toxoplasma gondii
Deposition date:
2020-03-11
Original release date:
2021-07-16
Authors:
Lin, Meng-Hsuan; Hsu, Chun-Hua
Citation:

Citation: Lin, Meng-Hsuan; Yu, Tsun-Ai; Chang, Chi-Fon; Nishikawa, Yoshifumi; Hsu, Chun-Hua. "NMR resonance assignments of the programmed cell death protein 5 (PDCD5) from Toxoplasma gondii"  Biomol. NMR Assign. 14, 277-280 (2020).
PubMed: 32578164

Assembly members:

Assembly members:
TgPDCD5, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts486
15N chemical shifts143
1H chemical shifts888

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TgPDCD51

Entities:

Entity 1, TgPDCD5 123 residues - Formula weight is not available

Residue S 0 is the remained amino acid after TEV protease cleavage.

1   SERMETGLNPROGLUGLUPHEALAALAALA
2   ALAARGGLYGLYPHEGLYGLYASPALAGLU
3   LYSALALYSALAALAGLUALAGLNGLUALA
4   METARGGLNGLNMETGLUGLUGLNARGARG
5   ILEMETLEUARGALAVALLEUTHRPROALA
6   ALAGLNGLUARGLEUHISARGILEGLNLEU
7   VALLYSALAASPLYSALAARGGLUVALGLU
8   ALALEUILELEUGLNASNALAGLNARGGLY
9   ARGLEUALAASPLYSVALASPGLUALATHR
10   LEUILEGLULEULEUGLNGLNTHRSERALA
11   ALASERALAALALYSASNTHRPROLYSVAL
12   THRMETARGARGARGPHESERASPASPASP
13   ASPASPPHE

Samples:

sample_1: TgPDCD5, [U-13C; U-15N], 0.4 mM; sodium phosphate 25 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 4.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks