BMRB Entry 28087

Name: dUnr Cterminal Qrich
Published: 2021-09-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28087

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

dUnr Cterminal Qrich

Deposition date:

2020-02-18

Original release date:

2021-09-21

Authors:

Hollmann, Nele Merret; Hennig, Janosch

Citation:

Citation: Hollmann, Nele Merret; Jagtap, Pravin Kumar Ankush; Masiewicz, Pawel; Guitart, Tanit; Simon, Bernd; Provaznik, Jan; Stein, Frank; Haberkant, Per; Sweetapple, Lara Jayne; Villacorte, Laura; Mooijman, Dylan; Benes, Valdimir; Savitski, Mikhail; Gebauer, Fatima; Hennig, Janosch. "Pseudo-RNA binding domains mediate RNA structure specificity in Upstream of N-Ras" Cell Rep. 32, 107930-107930 (2020).
PubMed: 32697992

Assembly members:

Assembly members:
dUNR_Cterminal_Q-rich, polymer, 85 residues, 8777.16 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dUNR_Cterminal_Q-rich: GQQQQQQQQQLHLNQSNAGA NINQNDQLGGLSNGISSSSS NASLQNGYVMHGSPGGSTSS VGSNNPVHLDEFKMENNNHA GSDAG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	116
15N chemical shifts	64
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dUnr Cterminal Q-rich	1

Entities:

Entity 1, dUnr Cterminal Q-rich 85 residues - 8777.16 Da.

The first residue (G) is a leftover from the cleavage site and not part of the native protein sequence.

1

GLY

GLN

2

LEU

HIS

LEU

ASN

GLN

SER

ASN

ALA

GLY

ALA

3

ASN

ILE

ASN

GLN

ASN

ASP

GLN

LEU

GLY

4

LEU

SER

ASN

GLY

ILE

SER

5

ASN

ALA

SER

LEU

GLN

ASN

GLY

TYR

VAL

MET

6

HIS

GLY

SER

PRO

GLY

SER

THR

SER

7

VAL

GLY

SER

ASN

PRO

VAL

HIS

LEU

ASP

8

GLU

PHE

LYS

MET

GLU

ASN

HIS

ALA

9

GLY

SER

ASP

ALA

GLY

Samples:

sample_1: dUNR Cterminal Q-rich, [U-99% 13C; U-99% 15N], 0.1 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

Cara, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance III 800 MHz

UNP	Q9VSK3
AlphaFold	Q9VSK3