BMRB Entry 28061

Title:
Backbone and side-chain chemical shift assignments of a cellular FLICE-inhibitory protein (c-FLIPs)
Deposition date:
2020-01-16
Original release date:
2021-01-25
Authors:
Bai, Zhiqiang; Liu, Bin; Ma, Xiaofang
Citation:

Citation: Bai, Zhiqiang; Liu, Bin; Ma, Xiaofang; Hu, Kaifeng. "Backbone and side-chain chemical shift assignments of a cellular FLICE-inhibitory protein (c-FLIPs)"  Biomol. NMR Assignments 14, 239-243 (2020).
PubMed: 32506385

Assembly members:

Assembly members:
c-FLPs, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts717
15N chemical shifts179
1H chemical shifts1208

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cellular FLICE-inhibitory protein short form1

Entities:

Entity 1, cellular FLICE-inhibitory protein short form 185 residues - Formula weight is not available

1   METGLYARGSERASPSERALAGLUVALILE
2   HISGLNVALGLUGLUALALEUASPTHRASP
3   GLULYSGLUMETLEULEUPHELEUCYSARG
4   ASPVALALAILEASPVALVALPROPROASN
5   VALARGASPLEULEUASPILELEUARGGLU
6   ARGGLYLYSLEUSERVALGLYASPLEUALA
7   GLULEULEUTYRARGVALARGARGPHEASP
8   LEULEULYSARGILELEULYSMETASPARG
9   LYSALAVALGLUTHRHISLEULEUARGASN
10   PROHISLEUVALSERASPTYRARGVALLEU
11   METALAGLUILEGLYGLUASPLEUASPLYS
12   SERASPVALSERSERLEUILEGLYLEUMET
13   LYSASPTYRMETGLYARGGLYLYSILESER
14   LYSGLULYSSERPHELEUASPLEUVALVAL
15   GLULEUGLULYSLEUASNLEUVALALAPRO
16   ASPGLNLEUASPLEULEUGLULYSCYSLEU
17   LYSASNILEHISARGILEASPLEULYSTHR
18   LYSILEGLNLYSTYRLYSGLNLEUGLUHIS
19   HISHISHISHISHIS

Samples:

c-FLIPs: c-FLPs, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 60 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 60 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCc-FLIPsisotropicsample_conditions_1
2D 1H-13C HSQCc-FLIPsisotropicsample_conditions_1
3D HNCAc-FLIPsisotropicsample_conditions_1
3D HNCACBc-FLIPsisotropicsample_conditions_1
3D CBCA(CO)NHc-FLIPsisotropicsample_conditions_1
3D HNCOc-FLIPsisotropicsample_conditions_1
3D HBHA(CO)NHc-FLIPsisotropicsample_conditions_1
3D HCCH-COSYc-FLIPsisotropicsample_conditions_1
3D 1H-15N NOESYc-FLIPsisotropicsample_conditions_1
3D 1H-13C NOESYc-FLIPsisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

NCBI NP_001338520.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks