BMRB Entry 28014

Name: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments of RimP from E. coli
Published: 2020-12-14

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PDB ID: 7afq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28014
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments of RimP from E. coli

Deposition date:

2019-09-11

Original release date:

2020-12-14

Authors:

Schedlbauer, Andreas; Kaminishi, Tatsuya; Iturrioz, Idoia; Ochoa Lizarralde, Borja; Gil-Carton, David; de Astigarraga, Elisa; Diercks, Tammo; Fucini, Paola; Connell, Sean

Citation:

Citation: Schedlbauer, Andreas; Ochoa-Lizarralde, Borja; Iturrioz, Idoia; Capuni, Retina; Diercks, Tammo; de Astigarraga, Elisa; Fucini, Paola; Connell, Sean. "Backbone and sidechain NMR assignments for the ribosome maturation factor RimP from Escherichia coli" Biomol. NMR Assign. 14, 189-193 (2020).
PubMed: 32303998

Assembly members:

Assembly members:
RimP, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p7XNH3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RimP: MSTLEQKLTEMITAPVEALG FELVGIEFIRGRTSTLRIYI DSEDGINVDDCADVSHQVSA VLDVEDPITVAYNLEVSSPG LDRPLFTAEHYARFVGEEVT LVLRMAVQNRRKWQGVIKAV DGEMITVTVEGKDEVFALSN IQKANLVPHFA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	619
15N chemical shifts	145
1H chemical shifts	991

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RimP	1

Entities:

Entity 1, RimP 151 residues - Formula weight is not available

1

MET

SER

THR

LEU

GLU

GLN

LYS

LEU

THR

GLU

2

MET

ILE

THR

ALA

PRO

VAL

GLU

ALA

LEU

GLY

3

PHE

GLU

LEU

VAL

GLY

ILE

GLU

PHE

ILE

ARG

4

GLY

ARG

THR

SER

THR

LEU

ARG

ILE

TYR

ILE

5

ASP

SER

GLU

ASP

GLY

ILE

ASN

VAL

ASP

6

CYS

ALA

ASP

VAL

SER

HIS

GLN

VAL

SER

ALA

7

VAL

LEU

ASP

VAL

GLU

ASP

PRO

ILE

THR

VAL

8

ALA

TYR

ASN

LEU

GLU

VAL

SER

PRO

GLY

9

LEU

ASP

ARG

PRO

LEU

PHE

THR

ALA

GLU

HIS

10

TYR

ALA

ARG

PHE

VAL

GLY

GLU

VAL

THR

11

LEU

VAL

LEU

ARG

MET

ALA

VAL

GLN

ASN

ARG

12

ARG

LYS

TRP

GLN

GLY

VAL

ILE

LYS

ALA

VAL

13

ASP

GLY

GLU

MET

ILE

THR

VAL

THR

VAL

GLU

14

GLY

LYS

ASP

GLU

VAL

PHE

ALA

LEU

SER

ASN

15

ILE

GLN

LYS

ALA

ASN

LEU

VAL

PRO

HIS

PHE

16

ALA

Samples:

sample_1: RimP, [U-98% 13C; U-98% 15N], 600 uM; HEPES 10 mM; MgCl2 6 mM; NH4Cl 30 mM; TCEP 75 uM

sample_conditions_1: pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CNH NOESY	sample_1	isotropic	sample_conditions_1
3D HNH NOESY	sample_1	isotropic	sample_conditions_1
3D HCH NOESY	sample_1	isotropic	sample_conditions_1
3D HNCAHA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CAHA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ccpnmr v2.4, CCPN - chemical shift assignment

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks