BMRB Entry 27975

Title:
Mouse Lemur Biliverdin B reductase
Deposition date:
2019-07-16
Original release date:
2020-09-28
Authors:
Eisenmesser, Elan; Redzic, Jasmina; Paukovich, Natasia
Citation:

Citation: Duff, Michael; Redzic, Jasmina; Ryan, Lucas; Paukovich, Natasia; Zhao, Rui; Nix, Jay; Pitts, Todd; Agarwal, Pratul; Eisenmesser, Elan Zohar. "Structure, dynamics and function of the evolutionarily changing biliverdin reductase B family"  J. Biochem. 168, 191-202 (2020).
PubMed: 32246827

Assembly members:

Assembly members:
Mouse_Lemur_BLVRB, polymer, 220 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: gray mouse lemur   Taxonomy ID: 30608   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Microcebus murinus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Data sets:
Data typeCount
13C chemical shifts331
15N chemical shifts178
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mouse Lemur BLVRB monomer1

Entities:

Entity 1, Mouse Lemur BLVRB monomer 220 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISLEUVAL
2   PROARGGLYSERMETALAVALLYSLYSILE
3   VALILEPHEGLYALATHRGLYARGTHRGLY
4   LEUTHRTHRLEUALAGLNALAVALGLNALA
5   GLYTYRGLUVALTHRVALLEUVALARGASP
6   SERSERARGLEUPROSERGLUGLYPROARG
7   PROALAARGVALILEVALGLYASPVALARG
8   HISALAALAASPVALASPLYSALAVALALA
9   GLYGLNASPALAVALVALVALLEULEUGLY
10   THRGLYASNASPLEUSERPROTHRTHRVAL
11   METSERGLUGLYALAARGASNILEVALALA
12   ALAMETLYSALAHISGLYVALASPLYSVAL
13   VALALACYSTHRSERALAPHELEULEUTRP
14   ASPPROALALYSVALPROPROARGLEUGLN
15   ASPVALTHRASPASPHISILEARGMETHIS
16   LYSVALLEUGLNGLUSERGLYLEULYSTYR
17   VALALAVALMETPROPROHISILEGLYASP
18   GLNPROLEUTHRGLYALATYRSERVALTHR
19   LEUASPGLYARGGLYPROSERARGVALILE
20   SERLYSHISASPLEUGLYHISPHEMETLEU
21   HISCYSLEUTHRTHRALAGLUTYRASPGLY
22   HISSERTHRTYRPROSERHISGLNTYRASP

Samples:

sample_1: Mouse Lemur BLVRB, [U-15N], 0.5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 20 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian DRX 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks