BMRB Entry 27948

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for ribosomal protein bL12 S89C and with LBT tag
Deposition date:
2019-06-17
Original release date:
2019-09-25
Authors:
Wang, Xiaolin; Kirkpatrick, John; Launay, Helene; de Simone, Alfonso; Haussinger, Daniel; Dobson, Christopher; Vendruscolo, Michele; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John
Citation:

Citation: Wang, Xiaolin; Kirkpatrick, John; Launay, Helene; de Simone, Alfonso; Haussinger, Daniel; Dobson, Christopher; Vendruscolo, Michele; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John. "Probing the dynamic stalk region of the ribosome using solution NMR"  Sci. Rep. 9, 13528-13528 (2019).
PubMed: 31537834

Assembly members:

Assembly members:
bL12_S89C, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli   Vector: pET21b(+)

Data sets:
Data typeCount
15N chemical shifts47
1H chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bL12, 11
2bL12, 21

Entities:

Entity 1, bL12, 1 121 residues - Formula weight is not available

1   METSERILETHRLYSASPGLNILEILEGLU
2   ALAVALALAALAMETSERVALMETASPVAL
3   VALGLULEUILESERALAMETGLUGLULYS
4   PHEGLYVALSERALAALAALAALAVALALA
5   VALALAALAGLYPROVALGLUALAALAGLU
6   GLULYSTHRGLUPHEASPVALILELEULYS
7   ALAALAGLYALAASNLYSVALALAVALILE
8   LYSALAVALARGGLYALATHRGLYLEUGLY
9   LEULYSGLUALALYSASPLEUVALGLUCYS
10   ALAPROALAALALEULYSGLUGLYVALSER
11   LYSASPASPALAGLUALALEULYSLYSALA
12   LEUGLUGLUALAGLYALAGLUVALGLUVAL
13   LYS

Samples:

sample_1: bL12 S89C LBT, [U-13C; U-15N], 0.9 mM; HEPES 10 mM; magnisium chloride 12 mM; ammonium chloride 30 mM; beta-mercaptoethanol 2 mM; DSS 0.001%

sample_conditions_1: ionic strength: 42 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1
3D HNCAsample_1anisotropicsample_conditions_1
3D HN(CO)CAsample_1anisotropicsample_conditions_1
3D HNCACBsample_1anisotropicsample_conditions_1

Software:

CcpNMR vVersion2.4, release 2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks