BMRB Entry 27930

Title:
Human Kibra WW domain chemical shift
Deposition date:
2019-05-29
Original release date:
2019-10-09
Authors:
Nyarko, Afua
Citation:

Citation: Kwok, Ethiene; Rodriguez, Diego; Kremerskothen, Joachim; Nyarko, Afua. "Intrinsic disorder and amino acid specificity modulate binding of the WW2 domain in kidney and brain protein (KIBRA) to synaptopodin"  J. Biol. Chem. 294, 17383-17394 (2019).
PubMed: 31597702

Assembly members:

Assembly members:
Kibra_WW_domain, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 24

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts116
15N chemical shifts70
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kibra WW domain1

Entities:

Entity 1, Kibra WW domain 95 residues - Formula weight is not available

1   GLYALAHISMETMETPROARGPROGLULEU
2   PROLEUPROGLUGLYTRPGLUGLUALAARG
3   ASPPHEASPGLYLYSVALTYRTYRILEASP
4   HISTHRASNARGTHRTHRSERTRPILEASP
5   PROARGASPARGTYRTHRLYSPROLEUTHR
6   PHEALAASPCYSILESERASPGLULEUPRO
7   LEUGLYTRPGLUGLUALATYRASPPROGLN
8   VALGLYASPTYRPHEILEASPHISASNTHR
9   LYSTHRTHRGLNILEGLUASPPROARGVAL
10   GLNTRPARGARGGLU

Samples:

sample_1: Kibra WW domain, [U-13C; U-15N], 180 uM

sample_conditions_1: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks